Effects of monoclonal antibodies to bovine and Paracoccus denitrificans cytochromes c on reactions with oxidase, reductase and peroxidase.
Effects of monoclonal antibodies to bovine and Paracoccus denitrificans cytochromes c on reactions with oxidase, reductase and peroxidase.
复制标题
牛和脱氮副球菌细胞色素 c 的单克隆抗体对氧化酶、还原酶和过氧化物酶反应的影响。
DOI：
10.1016/0005-2728(84)90263-9
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Smith,L
中科院分区：
文献类型：
--
作者：
Kuo,LM;Davies,HC;Smith,L
The effects of monoclonal antibodies to bovine andParacoccus denitrificanscytochromesc(Kuo, L.M. and Davies, H.C. (1983) Mol. Immunol. 20, 827–838) in the reactions of the cytochromescwith cytochromecoxidase, reductase and peroxidase were studied. Spectrophotometric assays were employed, under conditions where binding of cytochromecto the enzymes appears to be rate-limiting. Less than stoichiometric amounts of antibodies toP. denitrificanscytochromecadded to the cytochrome rendered some of it nonoxidizable or nonreducible by theP. denitrificansmembrane-bound electron transport system and decreased the rate constant with the remaining cytochromec. The antibodies appear to affect both electron transport reactions (blocking effects) with the oxidase and reductase and binding effects (effects on rate constants) and to distinguish between the two. Different ratios of antibody site to cytochromecgave different extents of blocking of the reductase as compared with the oxidase reaction. Differences were also apparent in the effect of these antibodies on the reaction of yeast peroxidase and the oxidase with theP. denitrificanscytochromec. Antibodies to bovine andP. denitrificanscytochromeschad considerably less effect on the reactions of the bovine cytochrome with bovine oxidase and reductase. One antibody was inhibitory to the oxidase reaction with bovine cytochromec, but not to that with the reductase. Also, an antibody which inhibited the oxidase reaction had no effect on the reaction with yeast peroxidase. The data give evidence that the interaction areas on cytochromecfor oxidase and reductase and peroxidase are not identical, although they may be nearby.
