A novel 40-kDa membrane-associated EF-hand calcium-binding protein in Plasmodium falciparum.
A novel 40-kDa membrane-associated EF-hand calcium-binding protein in Plasmodium falciparum.
复制标题
恶性疟原虫中一种新型 40 kDa 膜相关 EF 手钙结合蛋白。
DOI：
10.1016/s0021-9258(19)50621-4
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
David C. Kaslowg
中科院分区：
文献类型：
--
作者：
David J. RawlingsS;David C. Kaslowg
A 40-kDa sexual stage radiolabeled surface protein of Plasmodium falciparum, Pfs40, was previously identified as a potential target antigen of transmission blocking immunity by an immunogenetic approach. Synthetic oligonucleotide “guessmers,” based on microsequenced tryptic peptides of Pfs40 purified by two-dimensional gel electrophoresis, were used to clone the full length cDNA and genomic DNA encoding Pfs40. The deduced amino acid sequence predicted an integral membrane protein containing five EF-hand calcium-binding domains. The biological activity of one or more of these domains was confirmed by binding of 45Ca to both native and recombinant Pfs40. Antisera to recombinant Pfs40 immunoprecipitated the native radiolabeled 40-kDa surface protein. The predicted noncytosolic membrane-associated localization of Pfs40 is unique within the EF-hand calcium-binding protein superfamily.
