Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
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DOI：
10.1083/jcb.126.3.591
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发表时间：
1994-08
期刊：
影响因子：
--
通讯作者：
Wolffe AP
中科院分区：
文献类型：
--
作者：
Dimitrov S;Dasso MC;Wolffe AP
我们发现，重构的浓缩非洲爪蟾精子核的父原核卵提取物与磷酸化的核心组蛋白H2A，H2A.X和H4，和摄取的连接组蛋白B4和HMG 2蛋白。组蛋白B4是原核中染色体结构组装所必需的。然而，B4和核心组蛋白磷酸化都不需要间隔的核小体阵列的组装。我们认为，核小体阵列的间距是由相邻的组蛋白八聚体在生理组装条件下的相互作用。
We find that the remodeling of the condensed Xenopus laevis sperm nucleus into the paternal pronucleus in egg extracts is associated with phosphorylation of the core histones H2A, H2A.X and H4, and uptake of a linker histone B4 and a HMG 2 protein. Histone B4 is required for the assembly of chromatosome structures in the pronucleus. However neither B4 nor core histone phosphorylation are required for the assembly of spaced nucleosomal arrays. We suggest that the spacing of nucleosomal arrays is determined by interaction between adjacent histone octamers under physiological assembly conditions.