Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides
Protein transduction into the mouse otocyst using arginine-rich cell-penetrating peptides
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DOI：
10.1097/wnr.0b013e32834da8f8
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发表时间：
2011-12-21
期刊：
影响因子：
1.7
通讯作者：
Yumoto, Eiji
中科院分区：
文献类型：
--
作者：
Miwa, Toru;Minoda, Ryosei;Yumoto, Eiji
小鼠耳囊肿作为内耳的一种器官，是研究先天性内耳疾病的治疗方法和研究内耳发育的一个有吸引力的实验目标。聚精氨酸（6-12个残基）是一种细胞穿透肽，可用于将货物运送到细胞中。在这里，我们实现了将与9 -精氨酸肽融合的增强型绿色荧光蛋白（EGFP）经体外输送到小鼠胚胎耳囊中。注射后18 h，在耳囊内壁细胞及其附近均检测到EGFP信号。小鼠注射与九精氨酸肽融合的EGFP后，听觉和前庭功能正常。这些数据表明，使用聚精氨酸进行蛋白质转导可能是一种有用的替代策略，可以替代常用的基因传递方法，将治疗相关的分子传递到发育中的内耳。NeuroReport 22:994-999 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins。
The mouse otocyst, an anlage of the inner ear, is an attractive experimental target for developing treatment modalities for congenital inner ear diseases and for studying inner ear development. Poly-arginine (6-12 residues) is a cell-penetrating peptide and can be used to deliver cargo into cells. Here, we achieved transutero delivery of enhanced green fluorescent protein (EGFP) fused to a nine-arginine peptide into mouse embryonic otocysts. The EGFP signal was detected both in the lining cells of the otocysts and in their vicinity at 18 h post injection. Mice injected with EGFP fused to a nine-arginine peptide had normal auditory and vestibular functions. These data suggest that protein transduction using poly-arginine may be a useful alternative strategy to commonly used gene delivery methods for delivering therapeutically relevant molecules to the developing inner ear. NeuroReport 22:994-999 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.