Comparative analysis of the accelerated aged seed transcriptome profiles of two maize chromosome segment substitution lines
Comparative analysis of the accelerated aged seed transcriptome profiles of two maize chromosome segment substitution lines
复制标题
两个玉米染色体片段替换系加速老化种子转录组谱的比较分析
DOI：
10.1371/journal.pone.0216977
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Gu Riliang
中科院分区：
文献类型：
--
作者：
Li Li;Wang Feng;Li Xuhui;Peng Yixuan;Zhang Hongwei;Hey Stefan;Wang Guoying;Wang Jianhua;Gu Riliang
种子寿命是种子质量最基本的特征之一。两个染色体片段置换系，I178和X178，这表明在种子寿命的显着差异，进行转录组测序之前和之后5天的加速老化（AA）处理。与未老化处理相比，老化处理5天后，I178和X178中分别鉴定出286和220个差异表达基因（DEG）。在这些DEG中，在I178和X178中都检测到98个DEG，这些DEG在基因本体论（GO）术语中富集了核部分、细胞内部分、细胞器和膜的细胞成分。在碳水化合物衍生物分解代谢过程中，只有86个通常下调的基因在GO方面富集。此外，I178和X178中选择性剪接（AS）事件的转录组分析显示，63.6%的转录异构体在所有样品中发生AS，只有1.6%的转录异构体包含169个基因，这些基因在老化处理后表现出老化特异性AS。结合已报道的QTL定位结果，7个DEG在老化处理后表现为AS，定位区间内有13个DEG可能与种子寿命直接或间接相关。
Seed longevity is one of the most essential characteristics of seed quality. Two chromosome segment substitution lines, I178 and X178, which show significant differences in seed longevity, were subjected to transcriptome sequencing before and after five days of accelerated aging (AA) treatments. Compared to the non-aging treatment, 286 and 220 differentially expressed genes (DEGs) were identified after 5 days of aging treatment in I178 and X178, respectively. Of these DEGs, 98 were detected in both I178 and X178, which were enriched in Gene Ontology (GO) terms of the cellular component of the nuclear part, intracellular part, organelle and membrane. Only 86 commonly downregulated genes were enriched in GO terms of the carbohydrate derivative catabolic process. Additionally, transcriptome analysis of alternative splicing (AS) events in I178 and X178 showed that 63.6% of transcript isoforms occurred AS in all samples, and only 1.6% of transcript isoforms contained 169 genes that exhibited aging-specific AS arising after aging treatment. Combined with the reported QTL mapping result, 7 DEGs exhibited AS after aging treatment, and 13 DEGs in mapping interval were potential candidates that were directly or indirectly related to seed longevity.