THE 1ST 7 AMINO-ACIDS ENCODED BY THE V-SRC ONCOGENE ACT AS A MYRISTYLATION SIGNAL - LYSINE-7 IS A CRITICAL DETERMINANT
THE 1ST 7 AMINO-ACIDS ENCODED BY THE V-SRC ONCOGENE ACT AS A MYRISTYLATION SIGNAL - LYSINE-7 IS A CRITICAL DETERMINANT
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DOI：
10.1128/mcb.8.6.2435
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发表时间：
1988-06-01
影响因子：
5.3
通讯作者：
VARMUS, HE
中科院分区：
文献类型：
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作者：
KAPLAN, JM;MARDON, G;VARMUS, HE
劳斯肉瘤病毒的转化蛋白pp60v-src通过酰胺键与甘氨酸2共价偶联到肉豆蔻酸。肉豆蔻酰化促进pp60v-src与细胞膜的结合，这种亚细胞定位对于转化活性是必不可少的。本文的研究结果，结合其他人以前的报道，暗示由v-src编码的第七个氨基酸在肉豆蔻酰化反应中可能是重要的。天冬酰胺取代赖氨酸7大大降低了肉豆蔻酰化，膜协会，并转化活性的pp60v-src。相反，在残基7处的精氨酸取代对pp60v-src的这些性质中的任何一个都没有影响。添加由v-src编码的氨基酸1至7足以引起src-丙酮酸激酶融合蛋白的肉豆蔻酰化。我们的结论是，识别序列的豆蔻酰化pp60v-src包括氨基酸1至7和赖氨酸7是这个序列的关键组成部分。
The transforming protein of Rous sarcoma virus, pp60v-src, is covalently coupled to myristic acid by an amide linkage to glycine 2. Myristylation promotes the association of pp60v-src with cellular membranes, and this subcellular location is essential for transforming activity. The findings presented here, in conjunction with the previous reports of others, imply that the seventh amino acid encoded by v-src might be important in the myristylation reaction. Replacement of lysine 7 by asparagine greatly reduced the myristylation, membrane association, and transforming activity of pp60v-src. In contrast, substitution of arginine at residue 7 had no effect on any of these properties of pp60v-src. Addition of amino acids 1 to 7 encoded by v-src was sufficient to cause myristylation of a src-pyruvate kinase fusion protein. We conclude that the recognition sequence for myristylation of pp60v-src comprises amino acids 1 to 7 and that lysine 7 is a critical component of this sequence.