The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities.
The Bionanoprobe: hard X-ray fluorescence nanoprobe with cryogenic capabilities.
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DOI：
10.1107/s1600577513029676
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发表时间：
2014-01
影响因子：
2.5
通讯作者：
Vogt S
中科院分区：
文献类型：
--
作者：
Chen S;Deng J;Yuan Y;Flachenecker C;Mak R;Hornberger B;Jin Q;Shu D;Lai B;Maser J;Roehrig C;Paunesku T;Gleber SC;Vine DJ;Finney L;VonOsinski J;Bolbat M;Spink I;Chen Z;Steele J;Trapp D;Irwin J;Feser M;Snyder E;Brister K;Jacobsen C;Woloschak G;Vogt S
BIONANO探针用于研究冷冻水合生物体系中的微量元素，空间分辨率低于100 nm。这里展示了它的性能，并报告了第一个结果。硬X射线荧光显微镜是对整个细胞和组织等生物样品进行痕量元素分析的最灵敏的技术之一。传统的样品制备方法通常包括脱水，这会去除细胞内的水分，因此可能会导致结构坍塌，或者是包埋等侵入性过程。辐射引起的伪影也可能成为一个问题，特别是当空间分辨率增加超过亚微米尺度时。为了能够在接近自然状态的水合条件下成像，并减少结构辐射损伤，开发了生物探针(BNP)，这是一种具有低温样品环境和低温传输能力的硬X射线荧光纳米探针，致力于研究冷冻水合生物系统中的痕量元素。BNP安装在高级光子源第21扇区的波荡器光束线上。它为二维荧光成像提供了30 nm的空间分辨率。在第一次演示中，描述了仪器的设计和运动控制原理，量化了仪器的性能，并首次报道了在冷冻水合全细胞上使用BNP获得的结果。
The Bionanoprobe has been developed to study trace elements in frozen-hydrated biological systems with sub-100 nm spatial resolution. Here its performance is demonstrated and first results reported. Hard X-ray fluorescence microscopy is one of the most sensitive techniques for performing trace elemental analysis of biological samples such as whole cells and tissues. Conventional sample preparation methods usually involve dehydration, which removes cellular water and may consequently cause structural collapse, or invasive processes such as embedding. Radiation-induced artifacts may also become an issue, particularly as the spatial resolution increases beyond the sub-micrometer scale. To allow imaging under hydrated conditions, close to the ‘natural state’, as well as to reduce structural radiation damage, the Bionanoprobe (BNP) has been developed, a hard X-ray fluorescence nanoprobe with cryogenic sample environment and cryo transfer capabilities, dedicated to studying trace elements in frozen-hydrated biological systems. The BNP is installed at an undulator beamline at sector 21 of the Advanced Photon Source. It provides a spatial resolution of 30 nm for two-dimensional fluorescence imaging. In this first demonstration the instrument design and motion control principles are described, the instrument performance is quantified, and the first results obtained with the BNP on frozen-hydrated whole cells are reported.