Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.
Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.
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DOI：
10.15252/embj.2021107776
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发表时间：
2021-09-01
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen Z;Wang C;Feng X;Nie L;Tang M;Zhang H;Xiong Y;Swisher SK;Srivastava M;Chen J
宿主-病毒蛋白-蛋白相互作用在严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）的生命周期中起着关键作用。我们使用串联亲和纯化和邻近标记策略在病毒和宿主细胞之间进行了全面的相互作用组研究，并鉴定了437种人类蛋白质作为高置信度相互作用蛋白质。这些相互作用的进一步表征以及与其他对SARS-CoV-2感染的细胞反应的大规模研究的比较阐明了不同的SARS-CoV-2病毒蛋白如何参与其生命周期。通过这些数据挖掘，我们发现了治疗COVID-19的潜在药物靶点。将SARS-CoV-2编码的两种关键病毒蛋白NSP 1和N的相互作用组与其他人类冠状病毒中对应物的相互作用组进行了比较。这些比较不仅揭示了这些病毒为生存而操纵的共同宿主途径，而且还显示了不同的蛋白质-蛋白质相互作用，这可能解释了疾病病理学的差异。SARS-CoV-2的这种全面的相互作用组为理解和治疗这种疾病提供了宝贵的资源。被鉴定为与SARS-CoV-2蛋白共纯化或接近SARS-CoV-2蛋白的人类蛋白表明了细胞反应途径和潜在的药物靶点，以及与相关人类冠状病毒相比NSP 1和N蛋白结合特异性的差异。
Host–virus protein–protein interactions play key roles in the life cycle of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). We conducted a comprehensive interactome study between the virus and host cells using tandem affinity purification and proximity‐labeling strategies and identified 437 human proteins as the high‐confidence interacting proteins. Further characterization of these interactions and comparison to other large‐scale study of cellular responses to SARS‐CoV‐2 infection elucidated how distinct SARS‐CoV‐2 viral proteins participate in its life cycle. With these data mining, we discovered potential drug targets for the treatment of COVID‐19. The interactomes of two key SARS‐CoV‐2‐encoded viral proteins, NSP1 and N, were compared with the interactomes of their counterparts in other human coronaviruses. These comparisons not only revealed common host pathways these viruses manipulate for their survival, but also showed divergent protein–protein interactions that may explain differences in disease pathology. This comprehensive interactome of SARS‐CoV‐2 provides valuable resources for the understanding and treating of this disease. Human proteins identified as copurifying with or proximal to SARS‐CoV‐2 proteins suggest cellular response pathways and potential drug targets, as well as divergence of NSP1 and N protein binding specificities compared to related human coronaviruses.
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影响因子：
64.5
作者：
Prentzell MT;Rehbein U;Cadena Sandoval M;De Meulemeester AS;Baumeister R;Brohée L;Berdel B;Bockwoldt M;Carroll B;Chowdhury SR;von Deimling A;Demetriades C;Figlia G;Genomics England Research Consortium;de Araujo MEG;Heberle AM;Heiland I;Holzwarth B;Huber LA;Jaworski J;Kedra M;Kern K;Kopach A;Korolchuk VI;van 't Land-Kuper I;Macias M;Nellist M;Palm W;Pusch S;Ramos Pittol JM;Reil M;Reintjes A;Reuter F;Sampson JR;Scheldeman C;Siekierska A;Stefan E;Teleman AA;Thomas LE;Torres-Quesada O;Trump S;West HD;de Witte P;Woltering S;Yordanov TE;Zmorzynska J;Opitz CA;Thedieck K
通讯作者：
Thedieck K
DOI：
10.1126/science.abe9403
发表时间：
2020-12-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gordon DE;Hiatt J;Bouhaddou M;Rezelj VV;Ulferts S;Braberg H;Jureka AS;Obernier K;Guo JZ;Batra J;Kaake RM;Weckstein AR;Owens TW;Gupta M;Pourmal S;Titus EW;Cakir M;Soucheray M;McGregor M;Cakir Z;Jang G;O'Meara MJ;Tummino TA;Zhang Z;Foussard H;Rojc A;Zhou Y;Kuchenov D;Hüttenhain R;Xu J;Eckhardt M;Swaney DL;Fabius JM;Ummadi M;Tutuncuoglu B;Rathore U;Modak M;Haas P;Haas KM;Naing ZZC;Pulido EH;Shi Y;Barrio-Hernandez I;Memon D;Petsalaki E;Dunham A;Marrero MC;Burke D;Koh C;Vallet T;Silvas JA;Azumaya CM;Billesbølle C;Brilot AF;Campbell MG;Diallo A;Dickinson MS;Diwanji D;Herrera N;Hoppe N;Kratochvil HT;Liu Y;Merz GE;Moritz M;Nguyen HC;Nowotny C;Puchades C;Rizo AN;Schulze-Gahmen U;Smith AM;Sun M;Young ID;Zhao J;Asarnow D;Biel J;Bowen A;Braxton JR;Chen J;Chio CM;Chio US;Deshpande I;Doan L;Faust B;Flores S;Jin M;Kim K;Lam VL;Li F;Li J;Li YL;Li Y;Liu X;Lo M;Lopez KE;Melo AA;Moss FR 3rd;Nguyen P;Paulino J;Pawar KI;Peters JK;Pospiech TH Jr;Safari M;Sangwan S;Schaefer K;Thomas PV;Thwin AC;Trenker R;Tse E;Tsui TKM;Wang F;Whitis N;Yu Z;Zhang K;Zhang Y;Zhou F;Saltzberg D;QCRG Structural Biology Consortium;Hodder AJ;Shun-Shion AS;Williams DM;White KM;Rosales R;Kehrer T;Miorin L;Moreno E;Patel AH;Rihn S;Khalid MM;Vallejo-Gracia A;Fozouni P;Simoneau CR;Roth TL;Wu D;Karim MA;Ghoussaini M;Dunham I;Berardi F;Weigang S;Chazal M;Park J;Logue J;McGrath M;Weston S;Haupt R;Hastie CJ;Elliott M;Brown F;Burness KA;Reid E;Dorward M;Johnson C;Wilkinson SG;Geyer A;Giesel DM;Baillie C;Raggett S;Leech H;Toth R;Goodman N;Keough KC;Lind AL;Zoonomia Consortium;Klesh RJ;Hemphill KR;Carlson-Stevermer J;Oki J;Holden K;Maures T;Pollard KS;Sali A;Agard DA;Cheng Y;Fraser JS;Frost A;Jura N;Kortemme T;Manglik A;Southworth DR;Stroud RM;Alessi DR;Davies P;Frieman MB;Ideker T;Abate C;Jouvenet N;Kochs G;Shoichet B;Ott M;Palmarini M;Shokat KM;García-Sastre A;Rassen JA;Grosse R;Rosenberg OS;Verba KA;Basler CF;Vignuzzi M;Peden AA;Beltrao P;Krogan NJ
通讯作者：
Krogan NJ
影响因子：
64.5
作者：
Daniloski Z;Jordan TX;Wessels HH;Hoagland DA;Kasela S;Legut M;Maniatis S;Mimitou EP;Lu L;Geller E;Danziger O;Rosenberg BR;Phatnani H;Smibert P;Lappalainen T;tenOever BR;Sanjana NE
通讯作者：
Sanjana NE
DOI：
10.1073/pnas.0603144103
发表时间：
2006-08-22
影响因子：
11.1
作者：
Kamitani, Wataru;Narayanan, Krishna;Makino, Shinji
通讯作者：
Makino, Shinji
影响因子：
5.3
作者：
Davies, Jonathan P.;Almasy, Katherine M.;Plate, Lars
通讯作者：
Plate, Lars