Cognitive-enhancing effects of Rhus verniciflua bark extract and its active flavonoids with neuroprotective and anti-inflammatory activities
Cognitive-enhancing effects of Rhus verniciflua bark extract and its active flavonoids with neuroprotective and anti-inflammatory activities
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DOI：
10.1016/j.fct.2013.05.007
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发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
Sung, Sang Hyun
中科院分区：
文献类型：
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作者：
Cho, Namki;Lee, Ki Yong;Sung, Sang Hyun
类黄酮在大脑中的神经保护作用包括抗神经元细胞凋亡、抗神经炎症和增强认知功能。我们报道了漆树可抑制原代培养的大鼠皮质细胞中谷氨酸诱导的神经毒性。在这里，我们缩小范围，从富含类黄酮的乙酸乙酯成分 (PREF) 的植物中获取神经保护成分。在其活性黄酮类化合物中，非瑟酮不仅通过抑制诱导型一氧化氮合酶（iNOS）和环氧合酶（COX）-2来抑制脂多糖（LPS）诱导的神经炎症，而且通过东莨菪碱重新激活记忆受损小鼠的cAMP反应元件结合蛋白（CREB）-脑源性神经营养因子（BDNF）途径来增强记忆。紫紫黄素也表现出与非瑟酮相似的活性，尽管程度较小。 PREF 和选定的黄酮类化合物的神经保护作用可能涉及维持抗氧化防御机制，包括谷胱甘肽过氧化物酶 (GSH-Px)、谷胱甘肽还原酶 (GR) 和超氧化物歧化酶 (SOD)。最后，我们证明了 R. vernciflua 树皮提取物及其具有有效神经保护和抗炎作用的活性黄酮类化合物可能是作为认知增强剂的良好治疗候选者。 (C) 2013 Elsevier Ltd. 保留所有权利。
The neuroprotective potential of flavonoids within the brain comprises anti-apoptosis of neuronal cells, anti-neuroinflammation and enhancement of cognitive function. We reported that Rhus vernciflua inhibits glutamate-induced neurotoxicity in primary cultured rat cortical cells. Here we narrowed it down to get neuroprotective fractions from the plant yielding flavonoid-rich ethyl acetate fraction (PREF). Among its active flavonoids, fisetin exhibited not only inhibitory effect against lipopolysaccharide (LPS)-induced neuroinflammation by suppressing inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 but also memory enhancing effects via reactivation of cAMP responsive element binding protein (CREB)-brain derived neurotrophic factor (BDNF) pathway in memory-impaired mice by scopolamine. Butein also showed a similar activity to fisetin even though to a lesser extent. The neuroprotection by PREF and selected flavonoids may involve maintenance of antioxidant defense mechanism including glutathione peroxidase (GSH-Px), glutathione reductase (GR) and superoxide dismutase (SOD). Conclusively, we demonstrate the R. vernciflua bark extract and its active flavonoids with potent neuroprotective and anti-inflammatory effects might be good therapeutic candidates as cognitive-enhancers. (C) 2013 Elsevier Ltd. All rights reserved.