Ethylene-Responsive AP2/ERF Transcription Factor MACD1 Participates in Phytotoxin-Triggered Programmed Cell Death
Ethylene-Responsive AP2/ERF Transcription Factor MACD1 Participates in Phytotoxin-Triggered Programmed Cell Death
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DOI：
10.1094/mpmi-10-12-0253-r
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发表时间：
2013-08-01
影响因子：
3.5
通讯作者：
Yoshioka, Hirofumi
中科院分区：
文献类型：
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作者：
Mase, Keisuke;Ishihama, Nobuaki;Yoshioka, Hirofumi
为了研究植物细胞程序性死亡(PCD)，我们开发了植物毒素AAL的模拟系统，该毒素是由互隔交链格孢菌产生的。Lycopsici和对AAL敏感的美洲烟草。我们先前报道，乙烯(ET)信号在AAL触发的细胞死亡(ACD)中起着关键作用。然而，ET对ACD的下游信号转导机制尚不清楚。AAL细胞死亡调节因子1(MACD1)是一种APETALA2/ET反应因子(ERF)转录因子，参与ACD，并在ACD过程中作用于ET信号的下游。MACD1是一种转录激活剂，MACD1过表达植株比对照植株表现出更早的ACD诱导，这表明MACD1正向调节影响细胞死亡的因素。为了研究MACD1在PCD中的作用，我们使用了拟南芥和AAL的结构类似物伏马菌素B1(FB1)。在ET信号和erf102突变体中，FB1引发的细胞死亡受到影响。Loh2突变体对AAL敏感，loh2-1/erf102双突变体对ACD有抑制作用，表明ERF102也参与了ACD。为了研究ERF102调控的PCD相关基因，我们利用ERF102过表达植物的微阵列数据与AAL处理的loh2突变体上调基因数据库进行了比较，发现了ACD中受ERF102控制的基因。
To investigate plant programmed cell death (PCD), we developed the model system using phytotoxin AAL, which is produced by necrotrophic pathogen Alternaria alternata f. sp. lycopersici, and AAL-sensitive Nicotiana umbratica. We previously reported that ethylene (ET) signaling plays a pivotal role in AAL-triggered cell death (ACD). However, downstream signaling of ET to ACD remains unclear. Here, we show that the modulator of AAL cell death 1 (MACD1), which is an APETALA2/ET response factor (ERF) transcription factor, participates in ACD and acts downstream of ET signaling during ACD. MACD1 is a transcriptional activator and MACD1 overexpression plants showed earlier ACD induction than control plants, suggesting that MACD1 positively regulates factors affecting cell death. To investigate the role of MACD1 in PCD, we used Arabidopsis thaliana and a structural analog of AAL, fumonisin B1 (FB1). FB1-triggered cell death was compromised in ET signaling and erf102 mutants. The loh2 mutants showed sensitivity to AAL, and the loh2-1/erf102 double mutant compromised ACD, indicating that ERF102 also participates in ACD. To investigate the PCD-associated genes regulated by ERF102, we compared our microarray data using ERF102 overexpression plants with the database of upregulated genes by AAL treatment in loh2 mutants, and found genes under the control of ERF102 in ACD.