Exposure to toluene diisocyanate (TDI) induces IL-8 production from bronchial epithelial cells: effect of pro-inflammatory cytokines.
Exposure to toluene diisocyanate (TDI) induces IL-8 production from bronchial epithelial cells: effect of pro-inflammatory cytokines.
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接触甲苯二异氰酸酯 (TDI) 会诱导支气管上皮细胞产生 IL-8：促炎细胞因子的作用。
DOI：
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发表时间：
2003
影响因子：
4.5
通讯作者：
D. Nahm
中科院分区：
文献类型：
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作者：
Young;Hyun;Hae;S. Lee;D. Nahm
This investigation was designed to confirm IL-8 production from human bronchial epithelial cells with toluene diisocyanate (TDI) exposure and to examine the effects of pro-inflammatory cytokine and dexamethasone. We cultured Beas-2B, a bronchial epithelial cell line with TDI-HSA conjugate and compared with those without conjugate. IL-8 in the supernatant was measured by ELISA. To evaluate the effect of proinflammatory cytokines, peripheral blood mononuclear cells (PBMC) were collected from TDI- and non-TDI asthma patients, and were added to the epithelial cell culture. Dexamethasone or antibodies to TNF-alpha and IL-1beta were pre-incubated with PBMC supernatant. There was a significant production of IL-8 from bronchial epithelial cells with addition of TDI-HSA conjugate in a dose-dependent manner, which was significantly augmented with addition of PBMC supernatant. Higher production of IL-8 was noted with addition of PBMC supernatant from TDI-asthma patients than in those from non-TDI asthma patients. IL-1beta and IL-1beta/TNF alpha antibodies were able to suppress the IL-8 productions. Pre-treatment of dexamethasone induced dose-dependent inhibition of the IL-8 production. These results suggest that the IL-8 production from bronchial epithelial cells contribute to neutrophil recruitment occurring in TDI-induced airway inflammation. IL-1beta released from PBMC of TDI-induced asthma patients may be one of the pro-inflammatory cytokines to enhance IL-8 production.
异氰酸酯缀合的人肺上皮细胞蛋白：暴露与哮喘之间的联系？
DOI：
10.1016/s0091-6749(99)70377-5
发表时间：
1999
期刊：
The Journal of allergy and clinical immunology
影响因子：
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作者：
Wisnewski,AV;Lemus,R;Karol,MH;Redlich,CA
通讯作者：
Redlich,CA