An immunofluorescence procedure for the detection of intracellular immunoglobulins.
An immunofluorescence procedure for the detection of intracellular immunoglobulins.
复制标题
用于检测细胞内免疫球蛋白的免疫荧光程序。
DOI：
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发表时间：
1969
影响因子：
4.6
通讯作者：
F. Klein
中科院分区：
文献类型：
--
作者：
W. Hijmans;H. Schuit;F. Klein
本文给出了一种免疫荧光方法的技术细节，该方法是专门为研究细胞内免疫球蛋白而开发的，但也被证明可用于其他目的。
悬浮和洗涤细胞后，用沉降装置制备载玻片。结果是制备具有最小背景和在有限区域中的大量细胞的制剂。已发现这些制剂非常适合定性和定量研究。
该方法的描述还涉及偶联物专属性检测和显微照相。
This paper gives the technical details of an immunofluorescence procedure that was specially developed for the study of intracellular immunoglobulins but also proved useful for other purposes.
After suspending and washing of the cells, slides are prepared with a sedimentation apparatus. The result is a preparation with a minimal background and a large number of cells in a limited area. These preparations have been found to be eminently suitable for qualitative and quantitative studies.
The description of the method also deals with the testing of specificity of the conjugates and photomicrography.