F-spondin, expressed in somite regions avoided by neural crest cells, mediates inhibition of distinct somite domains to neural crest migration
F-spondin, expressed in somite regions avoided by neural crest cells, mediates inhibition of distinct somite domains to neural crest migration
复制标题
DOI：
10.1016/s0896-6273(00)80703-5
复制
发表时间：
1999-03-01
期刊：
影响因子：
16.2
通讯作者：
Kalcheim, C
中科院分区：
文献类型：
--
作者：
Debby-Brafman, A;Burstyn-Cohen, T;Kalcheim, C
神经嵴 (NC) 细胞专门迁移到每个巩膜切开器的吻侧半部，在那里它们避开皮肌切开器和旁索脊索巩膜切开器。 F-spondin 在这些抑制区域和整个尾半部表达。 NC 在头侧或尾侧上皮半体节（分别为 RS 或 CS）基底上扩散的体外生物测定表明，NC 细胞在 RS 上采用成纤维细胞形态，而在 CS 上它们无法变平。 F-spondin 抑制 RS 上 NC 细胞的扁平化。相反，F-spondin 抗体可防止 CS 上 NC 细胞的聚集。在树干外植体中添加 F-spondin 会抑制 NC 迁移到巩膜切开器中，并且用抗 F-spondin 抗体处理胚胎会导致迁移到其他抑制位点。因此，体节衍生的 F-spondin 是一种抑制信号，参与 NC 细胞的节段迁移及其在 RS 内的拓扑分离的模式化。
Neural crest (NC) cells migrate exclusively into the rostral half of each sclerotome, where they avoid the dermomyotome and the paranotochordal sclerotome. F-spondin is expressed in these inhibitory regions and throughout the caudal halves. In vitro bioassays of NC spreading on substrates of rostral or caudal epithelial-half somites (RS or CS, respectively) revealed that NC cells adopt on RS a fibroblastic morphology, whereas on CS they fail to flatten. F-spondin inhibited flattening of NC cells on RS. Conversely, F-spondin antibodies prevented rounding up of NC cells on CS. Addition of F-spondin to trunk explants inhibited NC migration into the sclerotome, and treatment of embryos with anti-F-spondin antibodies yielded migration into otherwise inhibitory sites. Thus, somite-derived F-spondin is an inhibitory signal involved in patterning the segmental migration of NC cells and their topographical segregation within the RS.