Activation of latent murine cytomegalovirus infection: cocultivation, cell transfer, and the effect of immunosuppression.
Activation of latent murine cytomegalovirus infection: cocultivation, cell transfer, and the effect of immunosuppression.
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潜伏鼠巨细胞病毒感染的激活：共培养、细胞转移和免疫抑制的作用。
DOI：
10.1093/infdis/138.6.890
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
M. Ho
中科院分区：
文献类型：
--
作者：
D. Mayo;J. Armstrong;M. Ho
小鼠巨细胞病毒感染16周以上，DBA/2小鼠各脏器未检出游离病毒。感染后6周，脾脏无游离溶解病毒。这类小鼠的脾细胞可通过三种方法潜伏感染。首先，在同基因或同种异体（CDI）胚胎成纤维细胞培养中，将脾细胞共培养两周或更长时间，恢复病毒。其次，从接受10(8)个脾细胞的同基因（DBA/2）或异基因（C57BL/10）小鼠的唾液腺中回收病毒。同种异体受体用环磷酰胺治疗可提高恢复，而用硫唑嘌呤治疗则没有。第三，潜伏感染的小鼠在接受环磷酰胺或硫唑嘌呤治疗后，其唾液腺中产生游离的小鼠巨细胞病毒。最后两项发现与免疫抑制患者和移植过程中接受潜伏感染细胞的患者中巨细胞病毒的观察结果相似。环磷酰胺和硫唑嘌呤均可提高唾液腺游离溶解病毒滴度。
No free virus was detectable in any organ of DBA/2 mice greater than or equal to 16 weeks after infection with murine cytomegalovirus. Spleens were free of free lytic virus six weeks after infection. Spleen cells from such mice were shown to be latently infected by three methods. First, virus was recovered by cocultivation of spleen cells for two weeks or longer on either syngeneic or allogeneic (CDI) embryonic fibroblast cultures. Second, virus was recovered from salivary glands of either syngeneic (DBA/2) or allogeneic (C57BL/10) mice that received 10(8) spleen cells. Recovery was enhanced by treatment of allogeneic recipients with cyclophosphamide but not with azathioprine. Third, latently infected mice, after treatment with either cyclophosphamide or azathioprine, developed free murine cytomegalovirus in their salivary glands. The last two findings parallel observations of human cytomegalovirus in immunosuppressed patients and in patients receiving latently infected cells during transplantation. Both cyclophosphamide and azathioprine elevated titers of free lytic virus in the salivary glands.