Verification of Sample Collection Sites for PCR-based Detection Assay for Abalone Asfa-like Virus
Verification of Sample Collection Sites for PCR-based Detection Assay for Abalone Asfa-like Virus
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DOI：
10.3147/jsfp.56.18
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发表时间：
2021-03-01
期刊：
影响因子：
0.6
通讯作者：
Nakayasu, Chihaya
中科院分区：
文献类型：
--
作者：
Matsuyama, Tomomasa;Kiryu, Ikunari;Nakayasu, Chihaya
采用定量聚合酶链式反应技术，测定了皱纹盘鲍(Haliotis Disus Disus)不同组织中鲍鱼ASFA样病毒(AbALV)的基因数量。在人体表面的任何组织或拭子中都检测到了大量的该基因。虽然血淋巴中的基因数量较低，但各组织间的基因数量没有显著差异。该病毒也是通过从受影响的动物身上进行常规PCR检测出来的。这些结果表明，这两种PCR都能检测到AbALV，破坏性试验在任何组织中都能检测到，非破坏性试验在体表粘液中也能检测到。
We measured gene quantities of the abalone asfa-like virus (AbALV), which is presumed to be the pathogen of abalone amyotrophia, in various tissues of affected Japanese abalone (Haliotis discus discus) with a quantitative PCR. Large quantities of the gene were detected in any tissues or swabs from the body surface. No significant difference in the gene quantity was found among tissues, although the gene quantity in the hemolymph was lower. The virus was also detected by a conventional PCR from affected animals. These results indicate that both PCRs are capable of the detection of AbALV, in any tissues by the destructive test and in body surface mucus by the non-destructive test.