Susceptibility to physiological concentrations of IL-1β varies in cartilage at different anatomical locations on human osteoarthritic knee joints
Susceptibility to physiological concentrations of IL-1β varies in cartilage at different anatomical locations on human osteoarthritic knee joints
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DOI：
10.1053/joca.2002.0515
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发表时间：
2002-04-01
影响因子：
7
通讯作者：
Westacott, CI
中科院分区：
文献类型：
--
作者：
Barakat, AF;Elson, CJ;Westacott, CI
目的：确定膝关节骨关节炎特定部位软骨组织对白细胞介素1β(IL-1β；1-10pg/ml)降解作用的敏感性是否存在差异，以确定软骨活检是否对IL-1β或肿瘤坏死因子α或两种分解代谢细胞因子的作用敏感。结果：同一关节不同部位和不同关节同一部位的活检组织对IL-1β和不同部位IL-1β作用的敏感性不同。对细胞因子有反应的区域数显著增加(P
Objectives: To determine whether cartilage biopsies from specific regions of osteoarthritic knee joints differ in susceptibility to the degradative effects of the amounts of interleukin-1beta (IL-1beta; 1-10 pg/ml) found in osteoarthritic joints, To establish whether biopsies are sensitive to the effects of either IL-1beta or TNFalpha or both catabolic cytokines.Methods: Cartilage from specified regions of 22 osteoarthritic knee joints was examined. Biopsies were incubated for 14 days without or with IL-1beta or TNFalpha at physiological concentrations and GAG release into supernatants assessed.Results: Variation was observed in susceptibility to the effects of 1-10 pg/ml IL-1beta in biopsies from different sites within the same joints and the same site in different joints. The number of regions responding to the cytokine increased significantly (P