The micelle to vesicle transition of lipids and detergents in the presence of a membrane protein: Towards a rationale for 2D crystallization
The micelle to vesicle transition of lipids and detergents in the presence of a membrane protein: Towards a rationale for 2D crystallization
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DOI：
10.1016/0014-5793(96)00180-9
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发表时间：
1996-03-11
期刊：
影响因子：
3.5
通讯作者：
Zulauf, M
中科院分区：
文献类型：
--
作者：
Dolder, M;Engel, A;Zulauf, M
用准弹性光散射和电子显微镜分析了脂质存在下二维膜蛋白晶体的组装。将洗涤剂溶解的脂质和/或蛋白质的混合物进行缓慢或快速稀释，同时测量聚集体的流体动力学半径。单独的脂质表现出中间棒状颗粒转化成小泡状的中间棒状稀释曲线。取决于蛋白质-蛋白质和蛋白质-脂质相互作用，洗涤剂溶解的蛋白质-脂质混合物显示出从胶束到大的、密集包装的蛋白脂质体的急剧转变。电子显微镜显示，晶体的形成后不久发生这种相变。
The assembly of two-dimensional membrane protein crystals in the presence of lipids was analyzed with quasielastic light scattering and electron microscopy, Mixtures of detergent-solubilized lipids and/or proteins were submitted to slow or rapid dilution while measuring the hydrodynamic radii of the aggregates. Lipids alone exhibited lambda-shaped dilution curves with intermediate rod-shaped particles that converted into small vesicles. Depending on the protein-protein and protein-lipid interactions, detergent-solubilized protein-lipid mixtures showed a sharp transition from micelles to large, densely packed proteoliposomes. Electron microscopy revealed that formation of crystals occurred shortly after this phase transition.