Production of tumor-associated antigen, TA-4, by the CaSki cervical carcinoma cell line.
Production of tumor-associated antigen, TA-4, by the CaSki cervical carcinoma cell line.
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CaSki 宫颈癌细胞系产生肿瘤相关抗原 TA-4。
DOI：
10.1097/00006250-198606000-00010
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发表时间：
1986
影响因子：
7.2
通讯作者：
Mochizuki,M
中科院分区：
文献类型：
--
作者：
Hussa,RO;Maruo,T;Strobel,JL;Pattillo,RA;Mochizuki,M
：之前的日本研究描述了从宫颈鳞状细胞癌切除的肿瘤组织匀浆中纯化 TA-4，以及开发放射免疫测定法来检测患有该疾病的患者血清中的 TA-4。本研究的目的是确定 TA-4 是否由 CaSki 细胞系产生，该细胞系十年前从宫颈表皮样癌培养中建立。放射免疫测定在 CaSki 细胞中检测到 TA-4 抗原，但在绒毛膜癌或乳腺癌衍生的细胞系中未检测到。 CaSki细胞裂解物中的TA-4浓度超过CaSki培养液中的浓度二十倍以上，并且超过人绒毛膜促性腺激素β样免疫反应物质的浓度近两个数量级。 TA-4 抗血清用于免疫沉淀来自与 [3H] 亮氨酸一起孵育的 CaSki 培养物的生物合成标记的 TA-4。电泳和放射自显影后，免疫沉淀材料显示出一条主带，其表观分子量（48,000 道尔顿）与最初从鳞状细胞癌肿瘤中分离的 TA-4 相对应。结论是，CaSki 细胞系构成了研究 TA-4 生物合成和调控的理想模型，也是大规模生产 TA-4 用于表征研究以及临床诊断试剂开发的来源。(Obstet Gynecol 67: 802, 1986)
: Previous Japanese studies described the purification of TA-4 from homogenates of tumor tissues excised from squamous cell carcinomas of the uterine cervix, and the development of a radioimmunoassay to detect TA-4 in sera of patients with this disease. The aim of the present investigation was to determine if TA-4 was produced by the CaSki cell line, established in culture ten years ago from epidermoid carcinoma of the uterine cervix. The radioimmunoassay detected the TA-4 antigen in the CaSki cells, but not in cell lines derived from either choriocarcinoma or breast carcinoma. The TA-4 concentration in the CaSki cell lysate exceeded that in the CaSki culture fluid by more than twentyfold, and exceeded the concentration of human chorionic gonadotropin [beta]-like immunoreactive material by nearly two orders of magnitude. Antiserum to TA-4 was used to immunoprecipitate biosynthetically labeled TA-4 from CaSki cultures that had been incubated with [3H] leucine. After electrophoresis and autoradiography, the immunoprecipitated material showed a major band corresponding in apparent molecular weight (48,000 daltons) to TA-4 originally isolated from squamous cell carcinoma tumors. It is concluded that the CaSki cell line constitutes an ideal model with which to investigate the biosynthesis and regulation of TA-4, and a source for large-scale production of TA-4 for characterization studies as well as development of clinical diagnostic reagents.(Obstet Gynecol 67: 802, 1986)