LOCATION OF PROGENITOR CELLS IN PERIODONTAL-LIGAMENT OF MOUSE MOLAR STIMULATED BY WOUNDING
LOCATION OF PROGENITOR CELLS IN PERIODONTAL-LIGAMENT OF MOUSE MOLAR STIMULATED BY WOUNDING
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DOI：
10.1002/ar.1091880202
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
BRUNETTE, DM
中科院分区：
文献类型：
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作者：
GOULD, TRL;MELCHER, AH;BRUNETTE, DM
在35 g小鼠下颌第一磨牙近中根上的牙槽突处创面，暴露牙周韧带。在受伤后3、5、7天，在死亡前3 h注射硫酸长春花碱，使分裂细胞处于中期。系列1 -.mu。用苏木精和伊红染色创面塑料切片M张。每4个切片测量有丝分裂象与最近的血管、骨和骨质之间的距离。不测量两次有丝分裂图。在每个伤口中测量至少500个间期细胞与最近血管的距离。各时期有丝分裂细胞的分布均比间期细胞更靠近血管（P < 0.01）。损伤后3 ~ 7 d，韧带骨侧有丝分裂活性明显改变，血管侧无明显改变（P < 0.05）。大约10%的有丝分裂细胞靠近牙骨质，但不靠近血管。多数分裂细胞为血管旁细胞，分2个群体，一个靠近骨，另一个位于韧带体。第三个种群，不是血管旁的，靠近骨质。
Wounds were made in the alveolar process overlying the mesial root of the mandibular 1st molar of 35-g mice to expose the periodontal ligament. Dividing cells were arrested in metaphase by injection of vinblastine sulfate 3 h before death 3, 5 and 7 days after wounding. Serial 1-.mu.m plastic sections of the wound were stained with hematoxylin and eosin. The distance between the mitotic figures and the nearest blood vessel, bone and cementum was measured in every 4th section. No mitotic figure was measured twice. The distances of at least 500 interphase cells from the closest blood vessel were measured in each wound. At all time periods the distribution of mitotic cells was significantly closer to blood vessels than that of interphase cells (P < 0.01). From 3 days to 7 days after wounding there was a significant shift of mitotic activity away from the bone side of the ligament, but not away from blood vessels (P < 0.05). Approximately 10% of all mitotic cells lay close to cementum, but not close to blood vessels. The majority of the dividing cells were confirmed to be paravascular, and appeared to belong to 2 populations, one adjacent to bone, the other in the body of the ligament. A 3rd population, not paravascular, lay adjacent to cementum.