Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase
Development and functional characterization of human bone marrow mesenchymal cells immortalized by enforced expression of telomerase
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DOI：
10.1046/j.1365-2141.2003.04217.x
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发表时间：
2003-03-01
影响因子：
6.5
通讯作者：
Campana, D
中科院分区：
文献类型：
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作者：
Mihara, K;Imai, C;Campana, D
为了建立永生的间充质细胞系，我们用端粒酶逆转录酶（TERT）转导原代人骨髓间充质细胞。TERT+间充质细胞持续生长> 2年;平行的TERT-培养物在15周后经历衰老。TERT+间充质细胞在软琼脂中不形成病灶，核型正常，可分化为成骨细胞和软骨细胞。它们支持白血病淋巴母细胞和正常CD 34（+）造血细胞的能力等于或大于原代细胞; 42个TERT+间充质细胞克隆的支持能力各不相同。永生化间充质细胞提供了一个很有前途的工具，以确定调节人类造血的分子。
To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34(+) haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis.