Maskless imaging of dense samples using pixel super-resolution based multi-height lensfree on-chip microscopy.
Maskless imaging of dense samples using pixel super-resolution based multi-height lensfree on-chip microscopy.
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DOI：
10.1364/oe.20.003129
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发表时间：
2012-01-30
期刊：
影响因子：
3.8
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Greenbaum A;Ozcan A
无透镜同轴全息显微镜在大视场范围内(例如，~24 mm2)提供亚微米分辨率，具有成本效益和紧凑的设计，适合现场使用。然而，它仅限于相对低密度的样品。为了缓解这一限制，我们展示了一种基于像素超分辨率和相位恢复的片上成像方法，该方法在多个无透镜强度测量之间迭代，每个测量具有略有不同的样本到传感器的距离。通过数字对准和配准这些无透镜强度测量，密集和连接的样品的相位和幅度图像可以在~24mm2的大视场内迭代重建，而不需要使用任何空间掩模。我们通过对致密的巴氏涂片(即巴氏涂片)和血液样本进行成像，展示了这种多高度串联全息方法的成功。
Lensfree in-line holographic microscopy offers sub-micron resolution over a large field-of-view (e.g., ~24 mm2) with a cost-effective and compact design suitable for field use. However, it is limited to relatively low-density samples. To mitigate this limitation, we demonstrate an on-chip imaging approach based on pixel super-resolution and phase recovery, which iterates among multiple lensfree intensity measurements, each having a slightly different sample-to-sensor distance. By digitally aligning and registering these lensfree intensity measurements, phase and amplitude images of dense and connected specimens can be iteratively reconstructed over a large field-of-view of ~24 mm2 without the use of any spatial masks. We demonstrate the success of this multi-height in-line holographic approach by imaging dense Papanicolaou smears (i.e., Pap smears) and blood samples.