Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3
Cloning of the promoter region of a human gene, FOXL2, and its regulation by STAT3
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人类基因 FOXL2 启动子区的克隆及其 STAT3 的调控
DOI：
10.3892/mmr.2017.6914
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发表时间：
2017-09-01
影响因子：
3.4
通讯作者：
Tang, Shengjian
中科院分区：
文献类型：
--
作者：
Han, Yangyang;Wang, Tianxiao;Tang, Shengjian
叉头盒L2（FOXL2）是一种转录因子，参与小睑裂、上睑下垂、内眦赘皮综合征（BPES）、卵巢早衰（POF）以及卵巢发育和功能的几乎所有阶段。FOXL2具有多种靶基因，其涉及许多过程，包括哺乳动物的性别决定、细胞周期调节、细胞凋亡和应激反应调节。然而，关于FOXL2的上游调控的研究是有限的。本研究成功克隆了FOXL2的启动子并在GenBank中注册，双荧光素酶报告基因（DLR）分析表明FOXL2的启动子对荧光素酶活性有显著的诱导作用。随后，生物信息学分析表明FOXL2可能受STAT3调节，并且这通过使用STAT3抑制剂的DLR分析和蛋白质印迹法证实。实时细胞分析进一步研究表明，HeLa细胞的活力显着抑制STAT3抑制剂。本研究证实了FOXL2表达上游调控的新发现，并为该领域的未来研究提供了新的视角。
Forkhead box L2 (FOXL2) is a transcription factor, which is involved in blepharophimosis, ptosis, and epicanthus in versus syndrome (BPES), premature ovarian failure (POF), as well as almost all stages of ovarian development and function. FOXL2 has various target genes, which are implicated in numerous processes, including sex determination, cell cycle regulation and apoptosis and stress response regulation in mammals. However, studies regarding the upstream regulation of FOXL2 are limited. In the present study, the promoter of FOXL2 was successfully cloned and registered in Gen Bank, and a dual luciferase reporter (DLR) analysis demonstrated that the luciferase activity was significantly induced by the promoter of FOXL2. Subsequently, bioinformatics analysis indicated that FOXL2 may be regulated by STAT3, and this was confirmed by a DLR analysis and western blotting, using STAT3 inhibitors. Further study using real-time cellular analysis indicated that the viability of He La cells was markedly suppressed by STAT3 inhibitors. The present study demonstrated novel findings regarding the upstream regulation of FOXL2 expression and provide a new perspective for future studies in the field.