Transcriptomic Analyses Indicate That Maize Ligule Development Recapitulates Gene Expression Patterns That Occur during Lateral Organ Initiation
Transcriptomic Analyses Indicate That Maize Ligule Development Recapitulates Gene Expression Patterns That Occur during Lateral Organ Initiation
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DOI：
10.1105/tpc.114.132688
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发表时间：
2014-12-01
期刊：
影响因子：
11.6
通讯作者：
Scanlon, Michael J.
中科院分区：
文献类型：
--
作者：
Johnston, Robyn;Wang, Minghui;Scanlon, Michael J.
多细胞生物的发育通过正确解释位置信息来建立边界，从而将具有不同身份的发育区域分开。玉米叶片是研究植物形态发生的理想系统，因为它被细分为近端叶鞘和远端叶片，每个都具有不同的发育模式。特化的叶舌和叶耳结构在叶鞘边界形成。叶耳作为一个铰链，允许叶片从茎上以一定角度突出，而叶舌包括一个表皮衍生的边缘。隐性无叶舌1突变体缺乏叶舌和叶耳，叶直立。我们使用激光显微切割和RNA测序，以确定差异表达的基因，在离散的细胞/组织特异性结构域沿着近端-远端轴的野生型叶原基经历叶舌启动和比较野生型和liguleless 1-R突变体叶原基的转录积累。我们确定了转录，特别是上调的叶片鞘边界。这些“叶舌基因”的数量也令人惊讶，已被证明在叶起始或侧支和交叉多个激素信号通路的功能。我们建议，在叶和/或分支启动利用的遗传模块重新部署，以调节叶舌从叶原基的生长。
Development of multicellular organisms proceeds via the correct interpretation of positional information to establish boundaries that separate developmental fields with distinct identities. The maize (Zea mays) leaf is an ideal system to study plant morphogenesis as it is subdivided into a proximal sheath and a distal blade, each with distinct developmental patterning. Specialized ligule and auricle structures form at the blade-sheath boundary. The auricles act as a hinge, allowing the leaf blade to project at an angle from the stem, while the ligule comprises an epidermally derived fringe. Recessive liguleless1 mutants lack ligules and auricles and have upright leaves. We used laser microdissection and RNA sequencing to identify genes that are differentially expressed in discrete cell/tissue-specific domains along the proximal-distal axis of wild-type leaf primordia undergoing ligule initiation and compared transcript accumulation in wild-type and liguleless1-R mutant leaf primordia. We identified transcripts that are specifically upregulated at the blade-sheath boundary. A surprising number of these "ligule genes" have also been shown to function during leaf initiation or lateral branching and intersect multiple hormonal signaling pathways. We propose that genetic modules utilized in leaf and/or branch initiation are redeployed to regulate ligule outgrowth from leaf primordia.