A cell cycle-regulated bacterial DNA methyltransferase is essential for viability
A cell cycle-regulated bacterial DNA methyltransferase is essential for viability
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DOI：
10.1073/pnas.93.3.1210
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发表时间：
1996-02-06
影响因子：
11.1
通讯作者：
Shapiro, L
中科院分区：
文献类型：
--
作者：
Stephens, C;Reisenauer, A;Shapiro, L
\ CcrM腺嘌呤DNA甲基转移酶，能够特异性修饰GANTC序列，是新月形根杆菌生存所必需的。据我们所知，这是第一个不属于DNA限制/修饰系统的基本原核DNA甲基转移酶的例子。CcrM的同源物在变形杆菌的α分支中广泛存在，这表明GANTC位点的甲基化可能在这个多样化群体的其他成员中也有重要的功能。UNA甲基化状态的时间控制在茎状杆菌的发育中起着重要作用，我们发现这种生物利用一种不同寻常的机制来控制新复制的DNA的再甲基化，CcrM在细胞周期的后期重新合成，与染色体的完全甲基化一致，然后在细胞分裂之前进行蛋白质水解。
\The CcrM adenine DNA methyltransferase, which specifically modifies GANTC sequences, is necessary for viability in Caulobacter crescentus, To our knowledge, this is the first example of an essential prokaryotic DNA methyltransferase that Is not part of a DNA restriction/modification system, Homologs of CcrM are widespread in the alpha subdivision of the Proteobacteria, suggesting that methylation at GANTC sites may have important functions in other members of this diverse group as well, Temporal control of UNA methylation state has an important role in Caulobacter development, and we show that this organism utilizes an unusual mechanism for control of remethylation of newly replicated DNA, CcrM is synthesized de novo late in the cell cycle, coincident with full methylation of the chromosome, and is then subjected to proteolysis prior to cell division.