Prominence of myosin heavy chain hybrid fibers in soleus muscle of spinal cord-transected rats.
Prominence of myosin heavy chain hybrid fibers in soleus muscle of spinal cord-transected rats.
复制标题
脊髓横断大鼠的比目鱼肌中肌球蛋白重链混合纤维的突出。
DOI：
10.1152/jappl.1995.78.4.1256
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Edgerton,VR
中科院分区：
文献类型：
--
作者：
Talmadge,RJ;Roy,RR;Edgerton,VR
研究了胸中部脊髓横断 (ST) 对大鼠比目鱼肌中肌球蛋白重链 (MHC) 亚型表达的影响。电泳分析表明，ST 后 15 天，IIx 型显着按比例增加，IIa 型减少，而 I 型 MHC 组成没有变化。 ST后30天，一些IIb型MHC表达，IIx型进一步成比例增加，IIa型增加（与15天ST相比），I型MHC减少。在 ST 后的两个时间段，许多纤维表达多种 MHC，如免疫组织化学所示，其中使用了一组针对 MHC 同工型的单克隆抗体。观察到纤维同时含有 I 型和 II 型或多种 II 型 MHC 同工型。这些数据表明，成年大鼠比目鱼肌中 MHC 同种型正常补体的表达部分依赖于正常的神经肌肉激活。
The effect of a midthoracic spinal cord transection (ST) on myosin heavy chain (MHC) isoform expression in the rat soleus muscle was studied. Electrophoretic analyses demonstrated that 15 days after ST there were significant proportional increases in type IIx, decreases in type IIa, and no change in type I MHC composition. Thirty days after ST, some type IIb MHC was expressed, there were further proportional increases in type IIx, an increase in type IIa (compared with 15-day ST), and a decrease in type I MHC. At both time periods after ST, many fibers expressed multiple MHCs, as demonstrated by immunohistochemistry where a battery of monoclonal antibodies specific to MHC isoforms was used. Fibers were observed containing types I and II together or multiple type II MHC isoforms. These data suggest that the expression of the normal complement of MHC isoforms in the adult rat soleus muscle is dependent, in part, on normal neuromuscular activation.