ECHS1 interacts with STAT3 and negatively regulates STAT3 signaling
ECHS1 interacts with STAT3 and negatively regulates STAT3 signaling
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DOI：
10.1016/j.febslet.2013.02.005
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发表时间：
2013-03-18
期刊：
影响因子：
3.5
通讯作者：
Zhang, Wei-Na
中科院分区：
文献类型：
--
作者：
Chang, Yan;Wang, Shao-Xin;Zhang, Wei-Na
信号换能器和转录激活因子3 （STAT3）是多种细胞过程中的关键转录因子，在一系列人类肿瘤中经常被过度激活。然而，调控STAT3激活的过程需要进一步阐明。通过酵母双杂交筛选，我们发现烯酰辅酶a水合酶短链1 （ECHS1）是一种新的STAT3结合蛋白。我们通过GST-pull - down和共降水进一步证实了STAT3和ECHS1之间的相互作用。重要的是，我们发现ECHS1特异性抑制STAT3活性，并通过抑制STAT3磷酸化负向调节STAT3几个靶基因的表达。因此，我们的研究结果将为STAT3信号调控的机制提供新的见解。蛋白质相互作用的结构化总结：STAT3通过下拉与ECHS1物理相互作用（View相互作用）STAT3通过两个杂交（View相互作用：1,2）ECHS1通过抗标签co免疫沉淀（View相互作用：1,2）STAT3通过抗诱饵co免疫沉淀（View相互作用）与ECHS1物理相互作用(C) 2013欧洲生化学会联合会。Elsevier B.V.版权所有。
Signal transducer and activator of transcription 3 (STAT3) is a critical transcriptional factor in a variety of cellular processes, and is frequently over-activated in a range of human tumors. However, the processes that regulate STAT3 activation need to be further clarified. With a yeast two-hybrid screening, we identified enoyl-CoA hydratase short chain 1 (ECHS1) as a novel STAT3 binding protein. We further confirmed the interaction between STAT3 and ECHS1 by GST-pull down and coimmnunoprecipitation. Importantly, we found that ECHS1 specifically represses STAT3 activity and negatively regulates the expression of several target genes of STAT3 through inhibiting STAT3 phosphorylation. Therefore, our findings will provide new insights into the mechanism of STAT3 signaling regulation.Structured summary of protein interactions:STAT3 physically interacts with ECHS1 by pull down (View interaction)STAT3 physically interacts with ECHS1 by two hybrid (View Interaction: 1, 2)ECHS1 physically interacts with STAT3 by anti tag co immunoprecipitation (View Interaction: 1, 2)STAT3 physically interacts with ECHS1 by anti bait co immunoprecipitation (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.