Insertion of a “chloroplast-like” regulatory segment responsible for thiol modulation into γ-subunit of F0F1-ATPase of the cyanobacterium Synechocystis 6803 by mutagenesis of atpC
Insertion of a “chloroplast-like” regulatory segment responsible for thiol modulation into γ-subunit of F0F1-ATPase of the cyanobacterium Synechocystis 6803 by mutagenesis of atpC
复制标题
通过 atpC 诱变将负责硫醇调节的“叶绿体样”调节片段插入蓝藻集胞藻 6803 的 F0F1-ATPase γ 亚基中
DOI：
10.1007/bf00283515
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
H. Strotmann
中科院分区：
文献类型：
--
作者：
S. Werner;Dorothea Gunkel;J. Schumann;H. Strotmann
高等植物叶绿体F0 F1-ATP酶复合体γ亚基中的调节序列负责所谓的巯基调节，在迄今为止分析的蓝藻复合体的相应多肽中不存在。我们已经通过定点突变修饰了集胞藻6803中编码该γ亚基的atpC基因。引入编码9个额外氨基酸的片段，包括两个功能性半胱氨酸，其构成叶绿体γ亚基中相应元件的序列。的生长速率以及光合作用的速率的突变体是可比的野生型，但短暂的呼吸增加后立即观察到一段时间的照明显着低于野生型的突变体。F1亚复合物溶解从类囊体的膜的野生型和的ESTA可以被激活的胰蛋白酶产生Ca 2+依赖的ATP酶活性，但只有F1从ESTA可以被激活的硫醇试剂二硫苏糖醇。
A regulatory sequence in the γ subunit of the F0F1-ATPase complex of higher plant chloroplasts, responsible for so-called thiol modulation, is absent in the corresponding polypeptides of the cyanobacterial complexes analysed so far. We have modified the atpC gene encoding this γ subunit in Synechocystis 6803 by site-directed mutagenesis. A segment was introduced coding for nine additional amino acids, including the two functional cysteines, which constitutes the sequence of the respective element in the chloroplast γ subunit. The growth rate as well as the rate of photosynthesis of the transformant was comparable to that of the wild-type, but the transitory increase in respiration observed immediately after a period of illumination was significantly lower in the mutant than in the wild-type. The F1 subcomplex solubilized from thylakoid membranes of both the wild-type and the transformant can be activated by trypsin to yield Ca2+-dependent ATPase activity, but only the F1 from the transformant can be activated by the thiol reagent dithiothreitol.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
蓝藻聚球藻中 6-磷酸葡萄糖酸脱氢酶和葡萄糖 6-磷酸脱氢酶的生长阶段依赖性诱导。
影响因子：
3.5
作者：
BroedelJr,SE;WolfJr,RE
通讯作者：
WolfJr,RE