BINDING OF SOLUBLE FORM OF FIBROBLAST SURFACE PROTEIN, FIBRONECTIN, TO COLLAGEN
BINDING OF SOLUBLE FORM OF FIBROBLAST SURFACE PROTEIN, FIBRONECTIN, TO COLLAGEN
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DOI：
10.1002/ijc.2910200102
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发表时间：
1977-01-01
影响因子：
6.4
通讯作者：
RUOSLAHTI, E
中科院分区：
文献类型：
--
作者：
ENGVALL, E;RUOSLAHTI, E
纤连蛋白是一种与正常成纤维细胞的主要表面蛋白在免疫学上相同的血浆蛋白，其结合胶原和明胶。固相酶免疫测定法用于结合试验。胶原蛋白、明胶或各种对照蛋白质被吸附到塑料表面。使用与碱性磷酸酶缀合的纯化的纤连蛋白抗体检测纤连蛋白的结合。从成纤维细胞培养物[人成人皮肤成纤维细胞]获得的循环纤连蛋白和纤连蛋白均显示出与胶原和明胶的特异性结合。血浆在明胶上的亲和层析偶联到Sepharose上，以高产率得到了免疫学和免疫学纯的纤连蛋白。恶性转化的成纤维细胞缺乏表面纤连蛋白。这些发现表明，这可能导致缺乏对周围细胞间基质的锚定，这可能有助于恶性生长行为。
Fibronectin, a plasma protein immunologically identical with a major surface protein of normal fibroblasts, binds collagen and gelatin. A solid phase enzyme immunoassay was used for the binding tests. Collagen, gelatin or various control proteins were adsorbed to a plastic surface. Binding of fibronectin was detected using purified fibronectin antibodies conjugated to alkaline phosphatase. Circulating fibronectin and fibronectin obtained from fibroblast cultures [human adult skin fibroblast] both showed specific binding to collagen and gelatin. Preparative affinity chromatography of plasma on gelatin coupled to Sepharose gave electrophoretically and immunologically pure fibronectin in high yields. Malignantly transformed fibroblasts lack surface fibronectin. These findings suggest the possibility that this results in a lack of anchorage to the surrounding intercellular matrix, which could contribute to the malignant growth behavior.