Three-dimensional imaging of Toxoplasma gondii-host cell interactions within the parasitophorous vacuole.
Three-dimensional imaging of Toxoplasma gondii-host cell interactions within the parasitophorous vacuole.
复制标题
寄生液泡内弓形虫与宿主细胞相互作用的三维成像。
DOI：
10.1017/s143192760404084x
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ris,Hans
中科院分区：
文献类型：
--
作者：
Schatten,Heide;Ris,Hans
弓形虫（Toxoplasma gondii）是顶复门寄生虫的代表，通过非常规的运动机制侵入宿主细胞。在宿主细胞侵入过程中，它形成一个特殊的被膜包围的隔室，称为寄生泡。宿主细胞和寄生虫膜之间的相互作用是复杂的，最近的研究更详细地揭示了宿主细胞和寄生虫膜有助于形成寄生虫液泡。通过使用我们的一种新的标本制备技术，允许三维成像的厚切片内部细胞结构与高分辨率，低电压场发射扫描电子显微镜，我们能够可视化连续的结构相互作用的宿主细胞膜与寄生虫内的寄生虫空泡。纤维和管状材料从宿主细胞膜延伸并连接到寄生虫膜组件。寄生虫也产生了较短的突起。这些较短的细突起中的几个连接到宿主细胞膜的纤维材料。这种复杂的网络可用于修改寄生虫液泡膜，使寄生虫在受到保护免受宿主细胞攻击的同时，能够利用宿主细胞的营养物质。寄生虫和宿主细胞之间的结构相互作用经历时间依赖性的变化，分裂孔是连接寄生虫和宿主细胞的最突出的结构。裂变孔由未知性质的厚结构成分锚定在宿主细胞中。通过这种技术获得的新信息包括纤维和管状材料的结构细节，这些材料在寄生虫和宿主细胞之间是连续的，并且可以在三维空间中成像。我们提出这种技术作为一种工具，更充分地调查复杂的结构相互作用的宿主细胞和寄生虫居住在寄生虫空泡。
The protozoan parasiteToxoplasma gondiiis a representative of apicomplexan parasites that invades host cells through an unconventional motility mechanism. During host cell invasion it forms a specialized membrane-surrounded compartment that is called the parasitophorous vacuole. The interactions between the host cell and parasite membranes are complex and recent studies have revealed in more detail that both the host cell and the parasite membrane contribute to the formation of the parasitophorous vacuole. By using our a new specimen preparation technique that allows three-dimensional imaging of thick-sectioned internal cell structures with high-resolution, low-voltage field emission scanning electron microscopy, we were able to visualize continuous structural interactions of the host cell membrane with the parasite within the parasitophorous vacuole. Fibrous and tubular material extends from the host cell membrane and is connected to parasite membrane components. Shorter protrusions are also elaborated from the parasite. Several of these shorter fine protrusions connect to the fibrous material of the host cell membrane. The elaborate network may be used for modifications of the parasitophorous vacuole membrane that will allow utilization of nutrients from the host cell by the parisite while it is being protected from host cell attacks. The structural interactions between parasite and host cells undergo time-dependent changes, and a fission pore is the most prominent structure left connecting the parasite with the host cell. The fission pore is anchored in the host cell by thick structural components of unknown nature. The new information gained with this technique includes structural details of fibrous and tubular material that is continuous between the parasite and host cell and can be imaged in three dimensions. We present this technique as a tool to investigate more fully the complex structural interactions of the host cell and the parasite residing in the parasitophorous vacuole.