Induction by the guanosine analogue oxanosine of reversion toward the normal phenotype of K-ras-transformed rat kidney cells.
Induction by the guanosine analogue oxanosine of reversion toward the normal phenotype of K-ras-transformed rat kidney cells.
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发表时间：
1989-02
期刊：
影响因子：
11.2
通讯作者：
O. Itoh;S. Kuroiwa;S. Atsumi;K. Umezawa;T. Takeuchi;M. Hori
中科院分区：
文献类型：
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作者：
O. Itoh;S. Kuroiwa;S. Atsumi;K. Umezawa;T. Takeuchi;M. Hori
Oxanosine，一种鸟苷类似物抗生素，在34摄氏度（允许温度）下改变了大鼠肾细胞的“转化”形态，将温度敏感的K-ras基因整合到“正常”形态中。氧肌苷也增加了纤维连接蛋白的细胞含量到正常水平。在这些条件下的细胞具有较低水平的鸟嘌呤核苷酸和不稳定且较少棕榈酰化的Mr 21，000蛋白（K-ras基因产物，称为p21）。p21的这些变化与在39.5 ℃（非允许温度）下不含氧黄核苷培养的细胞中p21发生的变化相同。
Oxanosine, a guanosine analogue antibiotic, altered the "transformed" morphology of rat kidney cells integrating a temperature-sensitive K-ras gene into "normal" morphology at 34 degrees C, a permissive temperature. Oxanosine also increased the cellular content of fibronectin to the normal level. The cells under these conditions had lower levels of guanine nucleotides and unstable and less palmitylated Mr 21,000 protein (K-ras gene product, referred to as p21). These changes in p21 were the same as those which occurred to p21 in the cells cultured in the absence of oxanosine at 39.5 degrees C, a nonpermissive temperature.