A DIRECT DETERMINATION OF NUCLEAR DISTRIBUTION IN HETEROKARYONS OF ASPERGILLUS NIDULANS
A DIRECT DETERMINATION OF NUCLEAR DISTRIBUTION IN HETEROKARYONS OF ASPERGILLUS NIDULANS
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DOI：
10.1017/s0016672300009605
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发表时间：
1966-01-01
影响因子：
1.5
通讯作者：
ROPER, JA
中科院分区：
文献类型：
--
作者：
CLUTTERBUCK, AJ;ROPER, JA
本文对构巢曲霉的单倍体和二倍体同核体以及它们之间形成的异核体进行了细胞学研究。生长的有效区域延伸到殖民地生长边缘后约600 μ，并与尖端细胞基本一致。吖啶荧光给非选择性染色的所有细胞核，投影核面积，并在个别菌丝的分布，确定为同核和异核体。二倍体和单倍体细胞核的平均投影面积不同，但其大小分布的重叠排除了明确的分类个别细胞核在二倍体-单倍体异核体。然而，异核体的菌丝在核比率上是不同的。这一点和其他证据强烈支持异核体的维持和适应是通过菌丝间而不是菌丝内选择发生的。
A cytological study has been made, in Aspergillus nidulans, of haploid and diploid homokaryons and of heterokaryons formed between them. The effective region of growth was shown to extend about 600 u behind the growing edge of the colony and to correspond largely with the tip cells. Acridine fluorescence gave unselective staining of all nuclei; projected nuclear areas, and their distributions in individual hyphae, were determined for the homokaryons and heterokaryons. Diploid and haploid nuclei differ in mean projected area but the overlap of their size distributions precluded unequivocal classification of individual nuclei in diploid-haploid heterokaryons. However, it was shown that the hyphae of a heterokaryon differ in nuclear ratios. This, and other evidence strongly support the suggestion that heterokaryon maintenance and adaptation occur through interhyphal rather than intrahyphal selection.