Identification of Escherichia coli region III flagellar gene products and description of two new flagellar genes
Identification of Escherichia coli region III flagellar gene products and description of two new flagellar genes
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大肠杆菌 III 区鞭毛基因产物的鉴定和两个新鞭毛基因的描述
DOI：
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发表时间：
1984
影响因子：
3.2
通讯作者：
P. Matsumura
中科院分区：
文献类型：
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作者：
D. Bartlett;P. Matsumura
大肠杆菌中的区域III鞭毛基因涉及鞭毛的组装和旋转以及趋性。我们将来自λ弗拉转导噬菌体的flaB操纵子亚克隆到质粒pMK 2004上。在flaB位点发现了另外两个基因，我们将flaB基因细分为flaB 1、flaBII和flaBIII。先前定位于flaB的cheY抑制突变进一步定位于flaB 11（Parkinson et al.，J. Bacteriol. 155：265-274，1983）。到目前为止，由于这些基因的基因表达水平低，基因产物鉴定还不可能。鞭毛基因的过表达通过将flaB操纵子置于lacUV 5或tac启动子的控制下来完成。在微细胞表达系统中检测质粒编码的蛋白质。通过将flaB操纵子中的各种缺失和插入与补充特定鞭毛突变体和编码多肽的能力相关联，我们进行了以下基因产物分配：flaB 1，60千道尔顿; flaB 11，38千道尔顿; flaB 111，28千道尔顿; flaC，56千道尔顿; fla 0，16千道尔顿;和flaE，54千道尔顿。
Region III flagellar genes in Escherichia coli are involved with the assembly and rotation of the flagella, as well as taxis. We subcloned the flaB operon from a lambda fla transducing phage onto plasmid pMK2004. Two additional genes were found at the flaB locus, and we subdivided the flaB gene into flaB1, flaBII, and flaBIII. The cheY suppressor mutations which have previously been mapped to flaB were further localized to flaB11 (Parkinson et al., J. Bacteriol. 155:265-274, 1983). Until now, gene product identification has not been possible for these genes because of their low levels of gene expression. Overexpression of the flagellar genes was accomplished by placing the flaB operon under the control of the lacUV5 or tac promoters. Plasmid-encoded proteins were examined in a minicell expression system. By correlating various deletions and insertions in the flaB operon with the ability to complement specific flagellar mutants and code for polypeptides, we made the following gene product assignments: flaB 1, 60 kilodaltons; flaB 11, 38 kilodaltons; flaB111, 28 kilodaltons; flaC, 56 kilodaltons; fla0, 16 kilodaltons; and flaE, 54 kilodaltons.
DOI：
10.1146/annurev.bb.13.060184.000411
发表时间：
1984
期刊：
Annual review of biophysics and bioengineering
影响因子：
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作者：
Macnab,RM;Aizawa,S
通讯作者：
Aizawa,S