Taxonomic status of the Japanese triploid forms of Fasciola: comparison of mitochondrial ND1 and COI sequences with F. hepatica and F. gigantica.
Taxonomic status of the Japanese triploid forms of Fasciola: comparison of mitochondrial ND1 and COI sequences with F. hepatica and F. gigantica.
复制标题
日本片形吸虫三倍体的分类地位：线粒体 ND1 和 COI 序列与肝片吸虫和巨大片吸虫的比较。
作者：
T. Itagaki;K. Tsutsumi;Kikukatsu Ito;Y. Tsutsumi
由于在形态学上难以识别日本片形吸虫，需要额外的分类学标准。为了阐明肝片形吸虫、巨型片形吸虫和日本三倍体片形吸虫之间的遗传关系，我们比较了线粒体NADH脱氢酶1 （ND1）和细胞色素c氧化酶1 （COI）基因的核苷酸序列。ND1和COI序列的种内变异较低，除了1个巨型飞虱的ND1序列外。日本三倍体片形吸虫的ND1和COI序列与巨型板形吸虫的ND1和COI序列几乎相同，但与肝板形吸虫的ND1和COI序列不同。因此，日本的三倍体片形吸虫被认为属于巨型片形吸虫。
Because it is difficult to identify morphologically the Japanese forms of Fasciola, additional taxonomic criteria are required. In order to clarify the genetic relationships between Fasciola hepatica, Fasciola gigantica, and the Japanese triploid forms of Fasciola, we compared nucleotide sequences of mitochondrial NADH dehydrogenase subunit 1 (ND1) and cytochrome c oxidase subunit I (COI) genes. Intraspecific variation in the ND1 and COI sequences was low, except for 1 specimen of F. gigantica in the ND1 sequence. The ND1 and COI sequences of Japanese triploid forms of Fasciola were nearly identical to those found in F. gigantica but were different from those of F. hepatica. Thus, the Japanese triploid forms of Fasciola are thought to be categorized as F. gigantica.