Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae.
Prevalence and clonal distribution of pcpA, psrP and Pilus-1 among pediatric isolates of Streptococcus pneumoniae.
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DOI：
10.1371/journal.pone.0041587
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Muñoz-Almagro C
中科院分区：
文献类型：
--
作者：
Selva L;Ciruela P;Blanchette K;del Amo E;Pallares R;Orihuela CJ;Muñoz-Almagro C
Streptococcus pneumoniae is the leading cause of vaccine-preventable deaths globally. The objective of this study was to determine the distribution and clonal type variability of three potential vaccine antigens: Pneumococcal serine-rich repeat protein (PsrP), Pilus-1, and Pneumococcal choline binding protein A (PcpA) among pneumococcal isolates from children with invasive pneumococcal disease and healthy nasopharyngeal carriers. We studied by Real-Time PCR a total of 458 invasive pneumococcal isolates and 89 nasopharyngeal pneumococcal isolates among children (total = 547 strains) collected in Barcelona, Spain, from January 2004 to July 2010. pcpA, psrP and pilus-1 were detected in 92.8%, 51.7% and 14.4% of invasive isolates and in 92.1%, 48.3% and 18% of carrier isolates, respectively. Within individual serotypes the prevalence of psrP and pilus-1 was highly dependent on the clonal type. pcpA was highly prevalent in all strains with the exception of those belonging to serotype 3 (33.3% in serotype 3 isolates vs. 95.1% in other serotypes; P<.001). psrP was significantly more frequent in those serotypes that are less apt to be detected in carriage than in disease; 58.7% vs. 39.1% P<.001. Antibiotic resistance was associated with the presence of pilus-1 and showed a negative correlation with psrP. These results indicate that PcpA, and subsequently Psrp and Pilus-1 together might be good candidates to be used in a next-generation of multivalent pneumococcal protein vaccine.
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影响因子：
3.7
作者：
Harvey RM;Stroeher UH;Ogunniyi AD;Smith-Vaughan HC;Leach AJ;Paton JC
通讯作者：
Paton JC
影响因子：
3.1
作者：
Glover, David T.;Hollingshead, Susan K.;Briles, David E.
通讯作者：
Briles, David E.
影响因子：
3.2
作者：
Moscoso, Miriam;Garcia, Ernesto;Lopez, Rubens
通讯作者：
Lopez, Rubens
影响因子：
6.7
作者：
Hilleringmann M;Giusti F;Baudner BC;Masignani V;Covacci A;Rappuoli R;Barocchi MA;Ferlenghi I
通讯作者：
Ferlenghi I
DOI：
10.1073/pnas.0511017103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Barocchi, MA;Rie, J;Henriques-Normark, B
通讯作者：
Henriques-Normark, B