One-dimensional acoustic standing waves in rectangular channels for flow cytometry
One-dimensional acoustic standing waves in rectangular channels for flow cytometry
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DOI：
10.1016/j.ymeth.2012.02.013
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发表时间：
2012-07-01
期刊：
影响因子：
4.8
通讯作者：
Graves, Steven W.
中科院分区：
文献类型：
--
作者：
Suthanthiraraj, Pearlson P. Austin;Piyasena, Menake E.;Graves, Steven W.
流式细胞术已成为一种强大的分析工具，其应用范围从血液诊断到微球阵列上分子组装体的高通量筛选。然而，仪器的尺寸、成本、通量以及耗材的使用限制了它在世界资源匮乏地区的应用，也限制了它作为环境监测的一个组成部分以及对极罕见细胞群检测的应用。基于这些原因，需要有新技术来改善流式细胞术的尺寸以及性价比。其中一项这样的技术是利用声驻波，它能有效地将细胞和颗粒集中到流动通道的中心以便进行分析。这种方法最简单的形式是利用一维声驻波在矩形通道中聚焦颗粒。我们已经开发出一维声聚焦流动通道，它可以在简单的毛细管装置中制造，或者使用光刻和深度反应离子蚀刻技术轻松地进行微制造。图像和视频分析表明，这些通道能为传统的流式细胞术分析精确地聚焦单个流动的颗粒流和细胞流。此外，使用具有更高谐波的驻波以及并行的微制造通道被证明能有效地产生许多并行的聚焦流。而且，我们介绍了一种用于矩形通道中流式细胞术的廉价光学平台的制造，以及该系统用于提供精确分析的情况。这里所开发的声聚焦装置的简单性和低成本有望使流式细胞仪在尺寸、成本和耗材使用方面都得以降低。最后，利用一维多节点声聚焦实现并行流动流的直接途径表明，矩形通道中的简单声聚焦在高通量流式细胞术中可能也具有重要作用。（C）2012爱思唯尔公司。保留所有权利。
Flow cytometry has become a powerful analytical tool for applications ranging from blood diagnostics to high throughput screening of molecular assemblies on microsphere arrays. However, instrument size, expense, throughput, and consumable use limit its use in resource poor areas of the world, as a component in environmental monitoring, and for detection of very rare cell populations. For these reasons, new technologies to improve the size and cost-to-performance ratio of flow cytometry are required. One such technology is the use of acoustic standing waves that efficiently concentrate cells and particles to the center of flow channels for analysis. The simplest form of this method uses one-dimensional acoustic standing waves to focus particles in rectangular channels. We have developed one-dimensional acoustic focusing flow channels that can be fabricated in simple capillary devices or easily microfabricated using photolithography and deep reactive ion etching. Image and video analysis demonstrates that these channels precisely focus single flowing streams of particles and cells for traditional flow cytometry analysis. Additionally, use of standing waves with increasing harmonics and in parallel microfabricated channels is shown to effectively create many parallel focused streams. Furthermore, we present the fabrication of an inexpensive optical platform for flow cytometry in rectangular channels and use of the system to provide precise analysis. The simplicity and low-cost of the acoustic focusing devices developed here promise to be effective for flow cytometers that have reduced size, cost, and consumable use. Finally, the straightforward path to parallel flow streams using one-dimensional multinode acoustic focusing, indicates that simple acoustic focusing in rectangular channels may also have a prominent role in high-throughput flow cytometry. (C) 2012 Elsevier Inc. All rights reserved.