A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S-cerevisiae
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S-cerevisiae
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DOI：
10.1016/s0092-8674(01)80008-8
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发表时间：
1997-10-03
期刊：
影响因子：
64.5
通讯作者：
Strunnikov, A
中科院分区：
文献类型：
--
作者：
Guacci, V;Koshland, D;Strunnikov, A
酿酒酵母MCD1（有丝分裂染色体决定因素）基因在遗传筛选中鉴定出对染色体结构重要的基因。 MCD1对于生存能力至关重要，并且从酵母到人类发现同源物。对MCD1P的MCD1突变体和细胞周期依赖性表达模式的分析表明，该蛋白在从S期通过有丝分裂的染色体形态发生起作用。 MCD1突变体在姐妹染色单体内聚力和染色体凝结中有缺陷。 MCD1P和SMC1P之间的物理关联是SMC染色体蛋白家族之一，进一步表明MCD1P直接在染色体上起作用。这些数据暗示MCD1P是内聚和凝结之间的联系。我们提出了一个有丝分裂染色体结构的模型，该模型结合了此先前未使用的链接。
The S. cerevisiae MCD1 (mitotic chromosome determinant) gene was identified in genetic screens for genes important for chromosome structure. MCD1 is essential for viability and homologs are found from yeast to humans. Analysis of the mcd1 mutant and cell cycle-dependent expression pattern of Mcd1p suggest that this protein functions in chromosome morphogenesis from S phase through mitosis. The mcd1 mutant is defective in sister chromatid cohesion and chromosome condensation. The physical association between Mcd1p and Smc1p, one of the SMC family of chromosomal proteins, further suggests that Mcd1p functions directly on chromosomes. These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link.