Dynamic monitoring of platelet deposition on severely damaged vessel wall in flowing blood. Effects of different stenoses on thrombus growth.
Dynamic monitoring of platelet deposition on severely damaged vessel wall in flowing blood. Effects of different stenoses on thrombus growth.
复制标题
动态监测流动血液中严重受损血管壁上的血小板沉积。
DOI：
10.1161/01.atv.10.2.306
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Badimon,L
中科院分区：
文献类型：
--
作者：
Lassila,R;Badimon,JJ;Vallabhajosula,S;Badimon,L
动脉血栓的形成是一个动态过程，取决于血流、触发基质和血液成分的特性。我们已经开发出一种灵敏和特异的计算机辅助核荧光成像方法，用于研究非狭窄和狭窄血流条件下体外和体内严重受损血管上血小板沉积的动力学。将具有铟-111标记的血小板的肝素化的猪血灌注50分钟。通过铟-111标记的转铁蛋白和铟-111标记的血小板评价静态和流动条件下的方法变异性。主要在血栓形成基质上存在严重高度狭窄时获得阳性血流图。由于该方法是高度敏感的，计算机辅助的轴向依赖性分析上的血管造影图像定位血栓积聚相对于狭窄的区域，并监测血小板积聚随时间的动态变化。在体外和体内，最高水平的血小板沉积发生在80%狭窄的顶点，通常可以在灌注30分钟后检测到栓塞。这项研究是第一个评估血栓在非平行流动流线中生长的动力学，例如在狭窄血管中遇到的。该方法为研究影响血栓形成和稳定性的因素提供了一种新的实验手段。
The formation of an arterial thrombus is a dynamic process that depends upon the characteristics of blood flow, the triggering substrate, and the blood components. We have developed and characterized a sensitive and specific computer-assisted nuclear scintigraphic method to study the dynamics of platelet deposition on severely damaged vessels both in vitro and in vivo in nonstenotic and stenotic flow conditions. Heparinized pig blood with Indium-111-labeled platelets was perfused for 50 minutes. Method variability in both static and flowing conditions was evaluated by Indium-111-labeled transferrin and Indium-111-labeled platelets. Positive scintigrams were obtained mainly in the presence of severe high grade stenoses on a thrombogenic substrate. Since the method is highly sensitive, computer-assisted axial dependence analysis was performed on the scintigraphic images to locate the thrombotic accumulation with respect to the area of the stenosis and to monitor the dynamic changes in platelet accumulation over time. Both in vitro and in vivo the highest level of platelet deposition occurred at the apex of the 80% stenosis, where embolization could be usually detected after 30 minutes of perfusion. This study is the first to assess the dynamics of thrombus growth in nonparallel flow streamlines such as are encountered in stenotic vessels. This method provides a new experimental tool with which to study factors affecting thrombus formation and stability.