Isolation and characterization of two alleles of the chicken cytochrome c gene.
Isolation and characterization of two alleles of the chicken cytochrome c gene.
复制标题
鸡细胞色素 c 基因的两个等位基因的分离和表征。
DOI：
10.1093/nar/11.24.8931
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发表时间：
1983
影响因子：
14.9
通讯作者：
Wu,R
中科院分区：
文献类型：
--
作者：
Limbach,KJ;Wu,R
通过基因组杂交对鸡的DNA进行分析，发现在鸡基因组中只存在一个细胞色素c基因。该细胞色素c基因的两个等位基因已从Charon 4a鸡基因组文库中分离出来。该分离利用酵母CYC1细胞色素c基因作为特异性杂交探针。对鸡的2个等位基因CC9和CC10进行了测序。这两个等位基因预测的氨基酸序列相同，与已发表的鸡细胞色素c蛋白序列一致。这两个等位基因的侧翼区域表现出大约1%的分化，表明多态性非常有限。与先前分离的细胞色素c基因（酵母和大鼠）的侧翼区域比较序列分析表明没有显著的同源区域。鸡基因组中仅存在一个细胞色素c样序列，与哺乳动物基因组中包含多达20-30个细胞色素c样序列形成鲜明对比。
Analysis of total chicken DNA by genomic blot hybridization indicates that only one cytochrome c gene exists in the chicken genome. The two alleles of this single cytochrome c gene have been isolated from a Charon 4A-chicken genomic library. This isolation made use of the yeast CYC1 cytochrome c gene as a specific hybridization probe. The 2 chicken alleles, CC9 and CC10, have been sequenced. The amino acid sequence predicted by these 2 alleles is identical, and agrees with the published chicken cytochrome c protein sequence. The flanking regions of these 2 alleles exhibit approximately 1% divergence, indicating a very limited polymorphism. Comparative sequence analysis with the flanking regions of previously isolated cytochrome c genes (yeast and rat) indicate no significant regions of homology. The presence of only one cytochrome c-like sequence in the chicken genome is in striking contrast with mammalian genomes, which contain as many as 20–30 cytochrome c-like sequences.