National survey for Salmonella in pigs, cattle and sheep at slaughter in Great Britain (1999-2000)
National survey for Salmonella in pigs, cattle and sheep at slaughter in Great Britain (1999-2000)
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DOI：
10.1111/j.1365-2672.2004.02192.x
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发表时间：
2004-01-01
影响因子：
4
通讯作者：
Teale, CJ
中科院分区：
文献类型：
--
作者：
Davies, RH;Dalziel, R;Teale, CJ
目的：这些调查的目的是估计健康猪、牛和羊在屠宰时粪便携带沙门氏菌的流行率，以及猪尸体被沙门氏菌污染的流行率。这些数据可用作基线，根据该基线可监测屠宰时这些物种中沙门氏菌流行率的未来变化。方法和结果：在英国屠宰猪、牛和羊中沙门氏菌粪便携带的首次随机全国调查中，在英格兰的34个猪屠宰场和117个红肉屠宰场对2509头猪、891头牛和973只羊进行了采样，苏格兰和威尔士。在578头（23.0%）猪的25 g盲肠内容物中发现沙门氏菌携带，但仅在134头（5.3%）尸体拭子中发现。在两种类型的样品中发现的主要沙门氏菌血清型是S。鼠伤寒（11.1%盲肠，2.1%尸体）;德比（6.3%盲肠，1.6%尸体）。S.发现的鼠伤寒杆菌为DT 104（占盲肠沙门氏菌的21.9%）。伤寒沙门氏菌分离株）、DT 193（18.7%）、未分型菌株（17.6%）、DT 208（13.3%）和U302（13.3%）。3株S.肠杆菌（PT 13 A和4）和1株恩诺沙星耐药S.还分离出了猪霍乱菌。在40%光密度（O.D.）下，从15.2%的猪中获得阳性的“肉汁ELISA”。临界值水平和10%临界值时的35.7%。阳性ELISA结果或尸体污染与盲肠携带沙门氏菌的相关性较差。尸体污染盲肠携带率的比例是最高的屠宰场从英格兰的米德兰地区和较小的屠宰场。在牛和羊中，检测了1 g直肠粪便样品。从牛中回收了两株分离株（即0.2%），分别为S.鼠伤寒沙门氏菌，DT 193和DT 12。一个绵羊样本（0.1%）含有沙门氏菌，S。鼠伤寒沙门氏菌DT 41。在一个小型的辅助验证活动中，使用来自174头牛样本的25 g直肠粪便，分离出3株（1.7%）沙门氏菌（S。鼠伤寒沙门氏菌DT 104、S. Agama，S.结论：与猪相比，英国主要屠宰牛和羊的沙门氏菌携带率很低。这表明，未来的控制措施应集中在减少沙门氏菌感染的猪场和尽量减少污染的尸体在slaughter.Significance和影响的研究：这项工作已经确定了基线数字沙门氏菌携带这些物种屠宰供人类消费在英国。这些数字是以具有代表性的方式收集的，使其能够用于监测趋势和制定控制目标。
Aims: The objective of these surveys was to estimate the prevalence of faecal carriage of Salmonella in healthy pigs, cattle and sheep at slaughter, and of pig carcase contamination with Salmonella. These data can be used as a baseline against which future change in Salmonella prevalence in these species at slaughter can be monitored.Methods and Results: In this first randomized National Survey for faecal carriage of Salmonella in slaughter pigs, cattle and sheep in Great Britain, 2509 pigs, 891 cattle and 973 sheep were sampled in 34 pig abattoirs and 117 red meat abattoirs in England, Scotland and Wales. Carriage of Salmonella in 25 g caecal contents was identified in 578 (23.0% pigs) but in only 134 (5.3%) of carcase swabs. The predominant Salmonella serovars found in both types of sample were S. Typhimurium (11.1% caeca, 2.1% carcases) and S. Derby (6.3% caeca, 1.6% carcases). The main definitive phage types (DT) of S. Typhimurium found were DT104 (21.9% of caecal S. Typhimurium isolates), DT193 (18.7%), untypable strains (17.6%), DT208 (13.3%) and U302 (13.3%). Three isolates of S. Enteritidis (PTs 13A and 4) and one enrofloxacin-resistant S. Choleraesuis were also isolated. A positive 'meat-juice ELISA' was obtained from 15.2% of pigs at 40% optical density (O.D.) cut-off level and 35.7% at 10% cut-off. There was poor correlation between positive ELISA results or carcase contamination and the caecal carriage of Salmonella. The ratio of carcase contamination to caecal carriage rates was highest in abattoirs from the midland region of England and in smaller abattoirs. In cattle and sheep 1 g samples of rectal faeces were tested. Two isolates (i.e. 0.2%) were recovered from cattle, one each of S. Typhimurium, DT193 and DT12. One sheep sample (0.1%) contained a Salmonella, S. Typhimurium DT41. In a small subsidiary validation exercise using 25 g of rectal faeces from 174 cattle samples, three (1.7%) isolates of Salmonella (S. Typhimurium DT104, S. Agama, S. Derby) were found.Conclusions: The carriage rate of Salmonella in prime slaughter cattle and sheep in Great Britain was very low compared with pigs. This suggests that future control measures should be focused on reduction of Salmonella infection on pig farms and minimizing contamination of carcases at slaughter.Significance and Impact of the Study: This work has set baseline figures for Salmonella carriage in these species slaughtered for human consumption in Great Britain. These figures were collected in a representative way, which enables them to be used for monitoring trends and setting control targets.