Single-Molecule and Superresolution Imaging in Live Bacteria Cells
Single-Molecule and Superresolution Imaging in Live Bacteria Cells
复制标题
DOI：
10.1101/cshperspect.a000448
复制
发表时间：
2010-03-01
影响因子：
7.2
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Biteen, Julie S.;Moerner, W. E.
单分子成像使生物物理测量不需要系综平均，提供了超过衍射极限的增强空间分辨率，并允许超分辨率重建。在这里，单分子和超分辨率成像被应用于研究活的新月藻细胞中的蛋白质，以说明这些方法在细菌成像中的能力。基于这些技术，我们在活细胞中以亚40 nm的空间分辨率研究了组氨酸蛋白激酶Plec的扩散系数和动力学、极性蛋白POPZ的定位行为以及结构蛋白MreB的踏步行为和蛋白质超结构。
Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions. Here, single-molecule and superresolution imaging are applied to the study of proteins in live Caulobacter crescentus cells to illustrate the power of these methods in bacterial imaging. Based on these techniques, the diffusion coefficient and dynamics of the histidine protein kinase PleC, the localization behavior of the polar protein PopZ, and the treadmilling behavior and protein superstructure of the structural protein MreB are investigated with sub-40-nm spatial resolution, all in live cells.