Strategies for three-dimensional particle tracking with holographic video microscopy
Strategies for three-dimensional particle tracking with holographic video microscopy
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DOI：
10.1364/oe.18.013563
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发表时间：
2010-06-21
期刊：
影响因子：
3.8
通讯作者：
Grier, David G.
中科院分区：
文献类型：
--
作者：
Cheong, Fook Chiong;Krishnatreya, Bhaskar Jyoti;Grier, David G.
在线全息显微镜捕获的视频流可以逐帧进行分析，以纳米分辨率跟踪单个胶体颗粒的三维运动。在这项工作中，我们比较了两种互补分析技术的性能，一种基于精确的 Lorenz-Mie 理论拟合，另一种基于对瑞利-索末菲反向传播重建的散射光场的现象学解释。尽管 Lorenz-Mie 跟踪提供了更多信息并且本质上更精确，但 Rayleigh-Sommerfeld 重建速度更快且更通用。这两种技术在胶体球的面内位置上定量一致。它们在轴向跟踪方面的系统差异可以用被照明物体的光散射特性来解释。 (C) 2010 美国光学学会
The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties. (C) 2010 Optical Society of America