Blood Species Identification for Forensic Purposes Using Raman Spectroscopy Combined with Advanced Statistical Analysis
Blood Species Identification for Forensic Purposes Using Raman Spectroscopy Combined with Advanced Statistical Analysis
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DOI：
10.1021/ac901350a
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Lednev, Igor K.
中科院分区：
文献类型：
--
作者：
Virkler, Kelly;Lednev, Igor K.
近年来，法医分析已成为分析化学发展最快的领域之一。确定体液样本来源物种的能力是法医调查的一个非常重要和关键的部分。本文介绍了一种新的技术，它利用现代分析方法的基础上相结合的拉曼光谱和先进的统计分析的血液痕迹，从不同物种的组成。近红外拉曼光谱（NIR）被用来分析多个干样品的人，狗，猫的血液最终应用于法医物种鉴定。所有的光谱被组合成一个单一的数据矩阵，并使用多种统计方法，如显着因子分析（SFA），主成分分析（PCA），和几个交叉验证方法，确定描述系统的主成分的数量。在确定存在的六个主成分中，前三个对系统光谱数据的贡献超过90%，用于形成三维分数图，该图清楚地显示了三组物种之间的显著分离。代表每个物种组周围99%置信区间的椭球体没有重叠。这种使用拉曼光谱的技术是非破坏性的和快速的，并且可以潜在地在犯罪现场进行。
Forensic analysis has become one of the most growing areas of analytical chemistry in recent years. The ability to determine the species of origin of a body fluid sample is a very important and crucial part of a forensic investigation. We introduce here a new technique which utilizes a modern analytical method based on the combination of Raman spectroscopy and advanced statistics to analyze the composition of blood traces from different species. Near-infrared Raman spectroscopy (NIR) was used to analyze multiple dry samples of human, canine, and feline blood for the ultimate application to forensic species identification. All of the spectra were combined into a single data matrix, and the number of principle components that described the system was determined using multiple statistical methods such as significant factor analysis (SFA), principle component analysis (PCA), and several cross-validation methods. Of the six principle components that were determined to be present, the first three, which contributed over 90% to the spectral data of the system, were used to form a three-dimensional scores plot that clearly showed significant separation between the three groups of species. Ellipsoids representing a 99% confidence interval surrounding each species group showed no overlap. This technique using Raman spectroscopy is nondestructive and quick and can potentially be performed at the scene of a crime.