A new application of nano-selenium: rescue of CK2 and mitochondria from oxidative stress to prevent cardiac hypertrophy.
A new application of nano-selenium: rescue of CK2 and mitochondria from oxidative stress to prevent cardiac hypertrophy.
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DOI：
10.2217/nnm-2022-0325
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发表时间：
2023-11
期刊：
影响因子：
5.5
通讯作者：
Xiaoqi Yang;Yang Fu;Jiaqi Liu;Jiabin Zhang;Xin Liu;Yuxuan Peng;Sandar Kyin;Mengdi Zhang;Donghai Zhou
中科院分区：
文献类型：
--
作者：
Xiaoqi Yang;Yang Fu;Jiaqi Liu;Jiabin Zhang;Xin Liu;Yuxuan Peng;Sandar Kyin;Mengdi Zhang;Donghai Zhou
背景：过多的活性氧(ROS)和随后的线粒体功能障碍是启动心肌肥厚的关键。为了探索纳米硒(SeNP)对这种情况的预防作用，作者在血管紧张素II(Ang II)诱导的心肌肥厚模型中评价了化学合成的壳聚糖-SeNPs和生物合成的蜡状芽孢杆菌YC-3-SeNPs。方法：包括ROS测定、线粒体膜电位分析、透射电子显微镜、基因和蛋白质表达分析、蛋白质羰化分析、血清抗氧化剂定量和组织学染色。结果：SeNPs通过降低ROS，恢复线粒体和蛋白激酶2α(CK2-α)功能，激活抗氧化途径，提高血清抗氧化水平，有效地对抗Ang II诱导的心肌肥厚。结论：这一发现强调了SeNPs在减轻Ang II诱导的体外和体内心肌肥厚中的作用。
Background: Excessive reactive oxygen species (ROS) and subsequent mitochondrial dysfunction are pivotal in initiating cardiac hypertrophy. To explore nano-selenium's (SeNP's) preventive potential against this condition, the authors evaluated chemically synthesized chitosan-SeNPs and biosynthesized Bacillus cereus YC-3-SeNPs in an angiotensin II (Ang II)-induced cardiac hypertrophy model. Methods: This investigation encompassed ROS measurement, mitochondrial membrane potential analysis, transmission electron microscopy, gene and protein expression analyses, protein carbonylation assays, serum antioxidant quantification and histological staining. Results: SeNPs effectively countered Ang II-induced cardiac hypertrophy by reducing ROS, restoring mitochondrial and protein kinase 2α (CK2-α) function, activating antioxidant pathways and enhancing serum antioxidant levels. Conclusion: This finding underscores SeNPs' role in attenuating Ang II-induced myocardial hypertrophy both in vitro and in vivo.