THE RECEPTOR-DESTROYING ENZYME OF V-CHOLERAE
THE RECEPTOR-DESTROYING ENZYME OF V-CHOLERAE
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DOI：
10.1038/icb.1947.33
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发表时间：
1947-01-01
期刊：
影响因子：
--
通讯作者：
STONE, JD
中科院分区：
文献类型：
--
作者：
BURNET, FM;STONE, JD
本文研究了霍乱弧菌产生的破坏红细胞“病毒受体”的酶的产生、滴定和部分纯化的条件。无论是在软琼脂平板上生长，还是通过在鸡胚的尿囊腔中培养弧菌，这种酶的产量都很高。本文介绍了一种快速滴定酶活力的方法。这种酶不耐热，在没有钙的情况下30分钟后就会完全被破坏。在52.5[℃]C加热，在IF/100 Ca存在下，热失活点提高到60[℃]C。酶在冷条件下能迅速吸附到红细胞上，最适pH约为9.2。如果这样的细胞在冷水中洗涤，酶随后在37摄氏度下洗脱。准备好了。可以获得不含大部分原始杂质的产品。
A study has been made of the conditions for production, titration and partial purification of the enzyme produced by Vibrio cholerae which destroys the "virus receptors" of red blood cells. The enzyme is produced in good yield either by growth on soft agar plates or by cultivation of the vibrio in the allantoic cavity of the chick-embryo. A method for rapid titration of enzyme activity is described. The enzyme is thermolabile, being completely destroyed in the absence of Ca after 30 mins''. heating at 52.5 [degree]C. In the presence of if/100 Ca the thermal inactivation point is raised to 60 [degree]C. Adsorption of enzyme to red cells takes place rapidly in the cold, the optimal pH for the reaction being about 9.2. If such cells are washed in the cold, and the enzyme subsequently allowed to elute at 37 [degree]C, conc. prepns. free of most of the original impurities can be obtained.