Fused Rocket Immunoelectrophoresis
Fused Rocket Immunoelectrophoresis
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融合火箭免疫电泳
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
P. J. Svendsen
中科院分区：
文献类型：
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作者：
N. Harboe;P. J. Svendsen
融合火箭免疫电泳是火箭免疫电泳的改进版本。将在分离实验中获得的级分或来自必须分析一种或多种蛋白质分布的其他实验的级分的等分试样转移到一排样品孔中，并使其扩散到无抗体的琼脂糖凝胶中（Harboe & Svendsen，未发表的观察，1969）。然后通过电泳将蛋白质强制进入含有抗体的琼脂糖凝胶中。必须针对参与上述实验的所有抗原产生抗体。因此，每种蛋白质的分布曲线将在相应的样品孔上显示为连续的沉淀线。
Fused rocket immunoelectrophoresis is a modified version of rocket immunoelectrophoresis. Aliquots of fractions obtained in separation experiments or of fractions from other experiments in which the distribution of one or more proteins must be analysed are transferred to a row of sample wells and are allowed to diffuse into an antibody-free agarose gel (Harboe & Svendsen, unpublished observation, 1969). The proteins are then by electrophoresis forced into an antibody-containing agarose gel. Antibodies must be raised against all antigens participating in the foregoing experiment. As a result, distribution profiles for each individual protein will appear as continuous precipitation lines over the corresponding sample wells.