Stimulation of collagenase production by collagen in mammalian cell cultures
Stimulation of collagenase production by collagen in mammalian cell cultures
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哺乳动物细胞培养物中胶原蛋白刺激胶原酶的产生
DOI：
10.1016/0092-8674(79)90216-2
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发表时间：
1979
期刊：
影响因子：
64.5
通讯作者：
J. Dayer
中科院分区：
文献类型：
--
作者：
C. Biswas;J. Dayer
在这项研究中，我们研究了可能的调节作用，胶原蛋白在胶原酶的生产培养的人皮肤成纤维细胞和人类和兔滑膜细胞。将I、II或III型胶原以溶液形式添加到培养基中显著刺激这些培养物中的胰蛋白酶可活化胶原酶活性。在人细胞培养物中，从人单核细胞培养基中分离的可溶性因子进一步增强了胶原蛋白的刺激作用（Dayer，Russell和Krane，1977）。天然和变性形式的胶原蛋白刺激酶的产生;它们的相对功效在不同类型之间变化。I型和II型胶原的天然形式显示出比相应的变性形式对胶原酶产生更大的影响，而对于III型胶原，变性形式更有效。
In this study, we investigated the possible regulatory role of collagen in collagenase production by cultured human skin fibroblasts and human and rabbit synovial cells. Addition of types I, II or Ill collagen in solution to the culture media markedly stimulated trypsin-activable coiiagenase activity in these cultures. In the human ceil cultures the stimulatory effect of collagen was further enhanced by a soluble factor isolated from human monocyte culture media (Dayer, Russell and Krane, 1977). Both native and denatured forms of collagen stimulated enzyme production; their relative efficacy varied among the different types. The native form of both types I and II collagen showed a greater effect on coiiagenase production than the corresponding denatured form, whereas with type ill collagen the denatured form was more effective.