Molecular characterization of mouse and rat CPP32 beta gene encoding a cysteine protease resembling interleukin-1 beta converting enzyme and CED-3.
Molecular characterization of mouse and rat CPP32 beta gene encoding a cysteine protease resembling interleukin-1 beta converting enzyme and CED-3.
复制标题
小鼠和大鼠 CPP32 β 基因的分子特征，该基因编码类似于 IL-1 β 转换酶和 CED-3 的半胱氨酸蛋白酶。
作者：
T. Juan;I. Mcniece;N. Jenkins;D. Gilbert;N. Copeland;F. Fletcher
白细胞介素-1 β转化酶（ICE）是一类新的哺乳动物半胱氨酸蛋白酶，与秀丽隐杆线虫死亡基因ced-3具有很强的同源性。ICE和CED-3在被引入培养细胞中时均诱导细胞凋亡，表明这种类型的半胱氨酸蛋白酶可能在程序性细胞死亡过程中起重要作用。在这里，我们报告的小鼠和大鼠的基因编码一种新的半胱氨酸蛋白酶的克隆。由这些cDNA编码的推定蛋白质含有与底物共价连接所必需的保守序列（QACRG）以及负责ICE中底物结合和催化的三个氨基酸。氨基酸序列分析表明，这种啮齿动物半胱氨酸蛋白酶是人CPP 32 β的同系物。小鼠CPP 32 β mRNA在脾脏中高度表达，在脑、肺、肝脏和肾脏中表达程度较低。小鼠CPP 32 β基因组基因座跨越约20 kb的区域，包括7个外显子和6个内含子。小鼠种间回交定位允许CPP 32 β定位于小鼠8号染色体的中心区域，与Scvr、Lpl、Jund 1和Mlr连锁。
Interleukin-1 beta converting enzyme (ICE) defines a new class of mammalian cysteine protease that shares strong homology with the Caenorhabditis elegans death gene ced-3. Both ICE and CED-3, when introduced into cultured cells, induce apoptosis, indicating that this type of cysteine protease may play an important role in the process of programmed cell death. Here, we report the cloning of a mouse and rat gene encoding a novel cysteine protease. The putative proteins encoded by these cDNAs contain the conserved sequence (QACRG) necessary for covalent linkage to the substrate as well as the three amino acids responsible for substrate binding and catalysis in ICE. Amino acid sequence analysis indicates that this rodent cysteine protease is the homolog of human CPP32 beta. Mouse CPP32 beta mRNA is highly expressed in spleen, and to a lesser degree in brain, lung, liver, and kidney. The mouse CPP32 beta genomic locus spans a region of approximately 20 kb, including seven exons and six introns. Mouse interspecific backcross mapping allowed localization of CPP32 beta to the central region of mouse chromosome 8, linked to Scvr, Lpl, Jund1 and Mlr.