Expression and localization of lumican in the ischemic and reperfused rat heart
Expression and localization of lumican in the ischemic and reperfused rat heart
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DOI：
10.1253/jcj.65.445
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发表时间：
2001-05-01
期刊：
影响因子：
--
通讯作者：
Asano, G
中科院分区：
文献类型：
--
作者：
Baba, H;Ishiwata, T;Asano, G
Lumican属于富含亮氨酸的小蛋白聚糖家族，在调节角膜和真皮胶原纤维组装中具有重要作用。最近，在心脏中发现了Lumican mRNA，并且据报道其在角膜伤口愈合期间的高表达。在本研究中，研究了Lumican在缺血和再灌注大鼠心脏纤维化中的表达和作用。在缺血再灌注大鼠心脏中，Lumican mRNA表达水平升高，第4周最高。对照组大鼠心脏中Lumican蛋白以核心蛋白和蛋白多糖的形式存在。在缺血再灌注的大鼠心脏中，4周时以蛋白多糖形式的Lumican的量增加，并且以糖蛋白形式的Lumican在缺血再灌注的大鼠心脏中被新检测到。在对照心脏中，Lumican在血管周围区域的胶原纤维中弱表达，但在第1天在缺血性病变的许多毛细血管内皮细胞中强烈表达。3天后：Lumican定位于胶原纤维和纤维化病变的成纤维细胞中。少数靠近缺血病变的心肌细胞表达Lumican mRNA。Lumican被认为在缺血再灌注大鼠心脏纤维形成中起重要作用。
Lumican belongs to the small leucine-rich proteoglycan family and has an important role in the regulation of corneal and dermal collagen fiber assembly. Recently, lumican mRNA was found in the heart and its high expression was reported during wound healing of the cornea. In the present study, the expression and role of lumican in fibrosis of an ischemic and reperfused rat heart were examined. The expression level of lumican mRNA increased in the ischemic and reperfused rat heart and was hi hest on the fourth week. Lumican protein existed in the forms of core protein and proteoglycan in the control rat heart. The amount of lumican in the form of proteoglycan increased, and that as the form glycoprotein was newly detected in the ischemic and reperfused rat heart on the fourth week. In the control heart, lumican was weakly expressed in the collagen fibers of the perivascular area, but it was expressed strongly in many capillary endothelial cells in the ischemic lesion on day 1. After 3 days: lumican was localized in collagen fibers and in the fibroblasts of fibrotic lesions. A few myocardial cells close to the ischemic lesion expressed lumican mRNA. Lumican is considered to play an important role in the fibrillogenesis of the ischemic and reperfused rat heart.