Association of genomic O-island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease
Association of genomic O-island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease
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DOI：
10.1128/jcm.41.11.4930-4940.2003
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发表时间：
2003-11-01
影响因子：
9.4
通讯作者：
Kaper, JB
中科院分区：
文献类型：
--
作者：
Karmali, MA;Mascarenhas, M;Kaper, JB
研究了EDL 933 O岛122(OI-122)在70株多种血清型VTEC中的分布情况，根据在人类疾病、暴发和/或溶血性尿毒症综合征(HUS)中出现的血清型，将VTEC分为5个“血清型”(A-E)。A型血清型包括10株O157：H7血清型和3株O157：NM血清型。B血清型(与暴发和HUS有关，但不太常见于O157：H7血清型)包括O26：H11、O103：H2、O111：NM、O121：H19和O145：NM三个血清型。血清型C型包括O91：H21和O113：H21血清型各4株，以及与散发性HUS有关但不典型地与暴发有关的8株其他血清型。血清型D型包括14株与腹泻有关但与暴发或HUS无关的血清型，而血清型E型包括与人类疾病无关的动物VTEC菌株。用聚合酶链式反应检测了4个EDL 933OI-122毒力基因(Z4321、Z4326、Z4332和Z4333)。Southern杂交证实为阴性。总体而言，28株(40%)菌株含有OI-122(对所有四种毒力基因都呈阳性)，27株(38.6%)含有“不完全”的OI-122(对一到三个基因呈阳性)，15株(21.4%)不含有OI-122。A型为100%，B型为60%，C型为36%，D型为15%，E型为0%。A型、B型和C型(与HUS相关)与D型和E型(与HUS无关)，A型和B型(与流行疾病相关)与C型、D型和E型(与流行疾病无关)之间01-122型的分布差异有高度显著性(P<；0.0001)。
The distribution of EDL 933 O island 122 (OI-122) was investigated in 70 strains of Verocytotoxin-producing Escherichia coli (VTEC) of multiple serotypes that were classified into five "seropathotypes" (A through E) based on the reported occurrence of serotypes in human disease, in outbreaks, and/or in the hemolytic-uremic syndrome (HUS). Seropathotype A comprised 10 serotype O157:H7 and 3 serotype O157:NM strains. Seropathotype B (associated with outbreaks and HUS but less commonly than serotype O157:H7) comprised three strains each of serotypes O26:H11, O103:H2, O111:NM, O121:H19, and O145:NM. Seropathotype C comprised four strains each of serotypes O91:H21 and O113:H21 and eight strains of other serotypes that have been associated with sporadic HUS but not typically with outbreaks. Seropathotype D comprised 14 strains of serotypes that have been associated with diarrhea but not with outbreaks or HUS, and seropathotype E comprised animal VTEC strains of serotypes not implicated in human disease. All strains were tested for four EDL 933 OI-122 virulence genes (Z4321, Z4326, Z4332, and Z4333) by PCR. Negative PCRs were confirmed by Southern hybridization. Overall, 28 (40%) strains contained OI-122 (positive for all four virulence genes), 27 (38.6%) contained an "incomplete" OI-122 (positive for one to three genes), and 15 (21.4%) strains did not contain OI-122. The seropathotype distribution of complete OI-122 was as follows: 100% for seropathotype A, 60% for B, 36% for C, 15% for D, and 0% for E. The differences in the frequency of 01-122 between seropathotypes A, B, and C (associated with HUS) and seropathotypes D and E (not associated with HUS) and between seropathotypes A and B (associated with epidemic disease) and seropathotypes C, D, and E (not associated with epidemic disease) were highly significant (P < 0.0001).