Intrauterine Growth Restriction Not Only Modifies the Cecocolonic Microbiota in Neonatal Rats But Also Affects Its Activity in Young Adult Rats
Intrauterine Growth Restriction Not Only Modifies the Cecocolonic Microbiota in Neonatal Rats But Also Affects Its Activity in Young Adult Rats
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DOI：
10.1097/mpg.0b013e3181d75d52
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发表时间：
2010-10-01
影响因子：
2.9
通讯作者：
Michel, Catherine
中科院分区：
文献类型：
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作者：
Fanca-Berthon, Pascale;Hoebler, Christine;Michel, Catherine
目的：阐明宫内生长受限(IUGR)易导致某些肠道病变的原因，有助于预防其发生。肠道微生物区系可能参与了这种易感性；IUGR可能会改变其初始设置，因为IUGR延迟了围产期肠道的发育，并与肠道生理强烈相互作用。此外，由于最初的定植决定成人肠道微生物区系，IUGR导致的初始微生物区系的缺陷将产生长期后果。因此，为了研究IUGR对肠道微生物区系的影响，我们比较了IUGR和未IUGR大鼠从出生到成年期的盲肠微生物区系的组成和活性。材料和方法：IUGR是通过限制妊娠等热量蛋白诱导的。幼崽由不受限制的哺乳期母亲喂养。在不同日龄(5、12、16、22、40和100d)时，对IUGR组和对照组大鼠的盲肠内容物进行细菌终产物浓度和主要菌群数量的分析，并进行体外发酵试验。结果：IUGR影响肠道定植：第5天细菌密度增加，第12天细菌密度下降。成年期IUGR大鼠仍与对照组不同，40d时双歧杆菌数量较少，100d时与月季杆菌相关的细菌较多。在体内，IUGR在断奶前降低了丙酸的浓度，而其他短链脂肪酸的浓度在40d时降低了，但体外代谢能力总体上没有受到影响。结论：IUGR本身导致了一些新生儿和持久的肠道微生物区系的变化。这些变化的生理后果以及它们与IUGR对肠道病理的易感效应的关系现在必须被探索。
Objective: Elucidating why intrauterine growth restriction (IUGR) predisposes to some intestinal pathologies would help in their prevention. Intestinal microbiota could be involved in this predisposition; its initial setup is likely to be altered by IUGR because IUGR delays perinatal intestinal development and strongly interacts with intestinal physiology. Furthermore, because initial colonization determines adult intestinal microbiota, an IUGR-induced defect in initial microbiota would have long-term consequences. Thus, to characterize the effect of IUGR on intestinal microbiota, we compared the composition and activity of cecocolonic microbiota from birth to adulthood in rats with and without IUGR.Materials and Methods: IUGR was induced by gestational isocaloric protein restriction. Pups were fed by unrestricted lactating mothers. At different ages (days 5, 12, 16, 22, 40, and 100), cecocolonic contents from rats with IUGR and controls were analyzed for concentrations of bacterial end products and numbers of main bacterial groups, and submitted to in vitro fermentation tests.Results: IUGR affected gut colonization: bacterial density was increased at day 5 and decreased at day 12. In adulthood, rats with IUGR still differed from controls, harboring fewer Bifidobacterium sp at day 40 and more bacteria related to Roseburia intestinalis at day 100. In vivo, propionate concentration was decreased by IUGR before weaning, whereas the concentrations of other short-chain fatty acids were decreased at day 40, although the in vitro metabolic capability was unaffected overall.Conclusions: We showed that IUGR induced, per se, some neonatal and long-lasting alterations of the intestinal microbiota. The physiological consequences of these changes and their relation to the predisposing effect of IUGR to gut pathologies must now be explored.